GC Selective Agar with LCAT

GC Selective Agar with LCAT

There are a number of media available for the selective isolation of Neisseria gonorrhoeae of which this is one. Based on the medium developed by Thayer & Martin, it includes Corn Starch to absorb toxic metabolites and is buffered to maintain a neutral pH. The medium is made selective by the inclusion of LCAT (Lincomycin, Colistin, Amphotericin B & Trimethoprim). Lincomycin and Colistin inhibit the growth of the majority of contaminating organisms likely to be present. Trimethoprim is included to reduce the spreading of Proteus spp and Amphotericin B to inhibit yeasts and fungi. Yeast Extract and Glucose are also added as further enrichment along with laked horse blood to ensure an more luxuriant growth of Neisseria gonorrhoeae.

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Organisms Ref. No Result
Neisseria gonorrhoeae NCTC 8375 Transparent/grey colonies
Neisseria gonorrhoeae NCTC 12700 Transparent/grey colonies
Candida albicans NCPF 3179 Inhibited
Staphylococcus epidermidis NCTC 13360 Inhibited
Proteus mirabilis NCTC 10975 Inhibited