There are a number of media available for the selective isolation of Neisseria gonorrhoeae of which this is one. Based on the medium developed by Thayer & Martin, it includes Corn Starch to absorb toxic metabolites and is buffered to maintain a neutral pH. The medium is made selective by the inclusion of LCAT (Lincomycin, Colistin, Amphotericin B & Trimethoprim). Lincomycin and Colistin inhibit the growth of the majority of contaminating organisms likely to be present. Trimethoprim is included to reduce the spreading of Proteus spp and Amphotericin B to inhibit yeasts and fungi. Yeast Extract and Glucose are also added as further enrichment along with laked horse blood to ensure an more luxuriant growth of Neisseria gonorrhoeae.
PP0780 – GC Selective Agar with LCAT
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Product Description
Organisms | Ref. No | Result |
Neisseria gonorrhoeae | NCTC 8375 | Transparent/grey colonies |
Neisseria gonorrhoeae | NCTC 12700 | Transparent/grey colonies |
Candida albicans | NCPF 3179 | Inhibited |
Staphylococcus epidermidis | NCTC 13360 | Inhibited |
Proteus mirabilis | NCTC 10975 | Inhibited |