GC Chocolate Agar with VCAT

GC Chocolate Agar with VCAT

This is one of a number of media available for the selective isolation of Neisseria gonorrhoeae. Based on the medium of Thayer & Martin it includes Corn Starch to absorb toxic metabolites and is buffered to maintain a neutral pH. The medium is enriched with Defibrinated Horse Blood where the blood has been ‘chocolated’ by heating the medium to 60°C and made selective by the inclusion of VCAT (Vancomycin, Colistin, Amphotericin and Trimethoprim). Vancomycin and Colistin inhibit the growth of the majority of contaminating organisms likely to be present. Trimethoprim is included to reduce the spread of Proteus spp and Amphotericin to suppress yeasts. Yeast Extract and Glucose are also added as further enrichment.

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Organisms Ref. No Result
Neisseria gonorrhoeae NCTC 8375 Transparent/grey colonies
Neisseria gonorrhoeae NCTC 12700 Transparent/grey colonies
Candida albicans NCPF 3179 Inhibited
Proteus mirabilis NCTC 10975 Inhibited
Staphylococcus epidermidis NCTC 13360 Inhibited