Middlebrook 7H11 Agar (25ml)

Middlebrook 7H11 Agar (25ml)

Middlebrooks 7H11 Selective Medium is an agar based medium for the isolation of Mycobacteria spp from clinical specimens. It differs from Middlebrooks 7H10 Agar in that it has a higher concentration of Malachite Green. The medium is complex and includes L-Glutamic Acid, Ammonium Sulphate, Sodium Citrate, Pyridoxine and Biotin as growth factors and Magnesium Sulphate, Ferric Ammonium Citrate as sources of trace ions. The medium is also made selective by the inclusion of Ticarcillin, Polymixin B, Trimethoprim and Amphotericin B. As with egg media Glycerol is included to enhance the growth of the Mycobacteria and Malachite Green is incorporated to provide a colour contrast between the colonies and the medium as well as contributing some inhibitory effect on organisms other than mycobacteria.

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Organisms Ref. No Result
Mycobacterium smegmatis NCTC 10265 Cream colonies with rough surface
Candida albicans NCPF 3179 Inhibited
Pseudomonas aeruginosa NCTC 12903 Inhibited
Aspergillus niger NCPF 2275 Inhibited
Staphylococcus aureus NCTC 12981 Inhibited