This is a modification of Sabouraud Dextrose Agar. The low pH (5.6) of the medium is inhibitory to most bacteria and it has been made specifically selective by the addition of colistin and gentamicin. This further reduces the risk of bacterial contamination when processing material that may be heavily contaminated particularly with Gram negative organisms such as Pseudomonas aeruginosa.
Sabouraud Dextrose Agar with Colistin (20mg/L) & Gentamicin (20mg/L)
|Shelf Life (days)|
|Storage Temp (°C)|
|Candida albicans||NCPF 3179||White colonies|
|Aspergillus brasiliensis||NCPF 2275||White/Yellow colonies on underside|
|Pseudomonas aeruginosa||NCTC 12903||Inhibited|
Recommended incubation: Yeasts – incubate Candida albicans aerobically at 25 ± 1°C for 48 hours. Fungi – incubate Aspergillus brasiliensis aerobically at 25 ± 1°C for 5-10 days. Bacteria – incubate Pseudomonas aeruginosa aerobically at 37 ± 1°C for 24-48 hours.