Wort Agar

Wort Agar is based on formulations developed by Parfitt (1933) and Scarr (1959), designed to replicate the composition of natural wort. Parfitt focused on yeast and mould counts in butter with Scarr’s later adaptation designed for growth of osmophilic yeast and their detection in sugar products and soft drinks. Wort Agar is primarily used for the cultivation, isolation, and enumeration of yeasts and moulds. It is especially effective for osmophilic yeasts that may be present in butter, sugar products, syrups, soft drinks, and sweet beverages. The low pH and presence of chloramphenicol inhibit bacterial growth.

Wort Agar can be used in compliance with ICUMSA (2022) Method GS2-47.

Additional Information

Container

Pack Size

Shelf Life (days)

Storage Temp (°C)

Appearance

Solid firm gel

Colour

Pale straw/grey

Recommended Incubation

Aerobically at 30 ± 1°C for 48 – 120 hours

pH

Volume

Product Description

Organisms Ref. No Result
Saccharomyces cerevisiae NCPF 3178 Growth: Cream colonies
Candida albicans NCPF 3179 Growth: Cream colonies
Aspergillus brasiliensis NCPF 2275 Growth: White colonies with black pigmentation
Mucor racemosus DSM 5266 Growth: White colonies with black pigmentation

Formula*

g/L
Malt extract 15.0
Peptone 0.78
Maltose 12.75
Dextrin 2.75
Di-potassium phosphate 1.0
Ammonium chloride 1.0
Agar 15.0
Glycerol 2.35
Chloramphenicol 0.1

*Adjusted/supplemented as needed to meet performance requirements.