Xylose Lysine Deoxycholate (XLD) Agar is used for the isolation and detection of Salmonella and Shigella spp. It differs from other media of this type in that it has less sodium deoxycholate as its selective agent. The indicator system is somewhat complex taking advantage of the fermentation or otherwise of three carbohydrates (lactose, sucrose and xylose) together with lysine decarboxylase and sodium thiosulphate as an indication of the presence or absence of hydrogen sulphide. The addition of novobiocin (20mg/L) improves the inhibition of Proteus spp.
Xylose Lysine Deoxycholate (XLD) Agar with Novobiocin (22.2mg/L)
|Shelf Life (days)|
|Storage Temp (°C)|
|Salmonella typhimurium||NCTC 12023||Growth, translucent colonies with black centre|
|Proteus mirabilis||NCTC 10975||Inhibited|
|Enterococcus faecalis||NCTC 12697||Inhibited|
|Escherichia coli||NCTC 12241||Inhibited|
Recommended incubation: Incubate aerobically at 37 ± 1°C for 24 ± 3 hours.