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E.E. Broth is recommended as an enrichment medium when examining food and feedstuffs for Enterobacteriaceae. It is a modification of Brilliant Green Bile Broth, with an improved buffering capacity to encourage early growth and prevent autosterilization. E.E. Broth uses glucose instead of lactose to make the medium a test for all enterobacteria including non-lactose fermenting organisms. This formulation complies with the Harmonized USP/EP/JP. -
E.E. Broth is recommended as an enrichment medium when examining food and feedstuffs for Enterobacteriaceae. It is a modification of Brilliant Green Bile Broth, with an improved buffering capacity to encourage early growth and prevent autosterilization. E.E. Broth uses glucose instead of lactose to make the medium a test for all enterobacteria including non-lactose fermenting organisms. This formulation complies with the Harmonized USP/EP/JP. Nitrogen is supplied by the gelatin peptone whilst glucose serves as the fermentable carbohydrate source. Oxbile and brilliant green are the selective agents helping to suppress Gram-positive non- target organisms. Auto sterilisation is prevented through the buffer system composed of potassium dihydrogen phosphate and disodium hydrogen phosphate.
