EE Broth (Mossel)

//EE Broth (Mossel)

EE Broth (Mossel)

SKU: KM0104 Category:

E.E. Broth is recommended as an enrichment medium when examining food and feedstuffs for Enterobacteriaceae.
It is a modification of Brilliant Green Bile Broth, with an improved buffering capacity to encourage early growth and prevent autosterilization.

E.E. Broth uses glucose instead of lactose to make the medium a test for all enterobacteria including non-lactose fermenting organisms.
This formulation complies with the Harmonized USP/EP/JP.

Nitrogen is supplied by the gelatin peptone whilst glucose serves as the fermentable carbohydrate source.
Oxbile and brilliant green are the selective agents helping to suppress Gram-positive non- target organisms.
Auto sterilisation is prevented through the buffer system composed of potassium dihydrogen phosphate and disodium hydrogen phosphate.

Additional Information


Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

OrganismRef. No.Result
Escherichia coliATTC 8739Turbid growth
Pseudomonas aeruginosaATCC 9027Turbid growth
Staphylococcus aureusATTC 6538Inhibited


Pancreatic digest of gelatin10
Potassium dihydrogen phosphate2
Disodium hydrogen phosphate8
Brilliant green0.15


Suspend 45.0 g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat at 100°C for 30 minutes and then cool immediately. DO NOT AUTOCLAVE. Aseptically dispense into the appropriate sterile containers.