E.E. Broth is recommended as an enrichment medium when examining food and feedstuffs for Enterobacteriaceae.
It is a modification of Brilliant Green Bile Broth, with an improved buffering capacity to encourage early growth and prevent autosterilization.
E.E. Broth uses glucose instead of lactose to make the medium a test for all enterobacteria including non-lactose fermenting organisms.
This formulation complies with the Harmonized USP/EP/JP.
Nitrogen is supplied by the gelatin peptone whilst glucose serves as the fermentable carbohydrate source.
Oxbile and brilliant green are the selective agents helping to suppress Gram-positive non- target organisms.
Auto sterilisation is prevented through the buffer system composed of potassium dihydrogen phosphate and disodium hydrogen phosphate.