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This is a medium supporting a wide range of organisms. It conforms to the requirements of the United States Pharmacopeia (USP) for antibiotic sensitivity testing of pharmaceutical products. As previously stated Antibiotic Medium No. 1 is used in the performance of antibiotic assays. This medium is prepared according to the specifications detailed in the USP. The use of this medium assures well-defined inhibition zones of the test organisms. Nutrients and growth factors are supplied by peptic digest of animal tissue, casein hydrolysate, yeast extract, and beef extract. Glucose is a carbon source.
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This is a medium supporting a wide range of organisms. It conforms to the requirements of the United States Pharmacopeia for antibiotic sensitivity testing of pharmaceutical products. Antibiotic Assay Medium No.32 is a modification of Antibiotic assay medium No.1. (E & O BM4710). This medium is used to develop inoculum of Bacillus subtilis for antibiotic assay used in the test for assaying by the plate assay method. Nutrients and growth factors are supplied by peptic digest of animal tissue, casein hydrolysate, yeast extract, and beef extract. Glucose is a carbon source.
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This is a medium supporting a wide range of organisms. It conforms to the requirements of the United States Pharmacopeia for antibiotic sensitivity testing of pharmaceutical products. Antibiotic Assay Medium No. 8 is recommended for preparing inoculum of Bacillus subtilis to be used as a test for assaying Vancomycin by plate assay method.
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Bile Aesculin Medium is generally used for the differentiation and presumptive identification of Group D streptococci (enterococci). Enterococci hydrolyse aesculin forming, amongst other products, aesculetin which in turn combines with Ferric ammonium citrate producing a dark brown or black complex. The presence of Bile salts in the medium inhibits gram positive organisms other than enterococci. The medium can also be used for the presumptive identification of certain organisms within the Enterobacteriaceae group such as Klebsiella spp., Enterobacter spp., etc.
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This is a very nutritious general-purpose medium suitable for the isolation of most organisms including many fastidious anaerobes. It is particularly recommended for streptococci and neisseria.
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A very nutritious isotonic general purpose medium with a low concentration of Glucose to stimulate early growth, Brain Heart Infusion Broth is suitable for the isolation of most micro-organisms including many fastidious organisms and, with enrichment as appropriate, is suitable as a base for blood culture medium. A phosphate buffer is incorporated to help neutralise any acids produced as a result of Glucose utilisation and thus maintain viability of the organisms. NB: Organisms that produce large amounts of acid in the medium may overwhelm the buffering system and as a result ‘auto-sterilise’ the culture.
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This medium is used to detect and/or confirm the presence of coli-aerogenes group of organisms in water, food and dairy laboratories. Bile and Brilliant Green are included in the medium to inhibit gram positive organisms while the coli-aerogenes organisms are identified by the formation of gas during the fermentation of Lactose. The medium can also be used for the confirmation of Escherichia coli by incubating at 44°C.
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Brucella Broth was developed to cultivate Brucella spp. from a wide variety of clinical samples but it is also widely used as a general enrichment broth for both fastidious and non-fastidious organisms.