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LB media formulations have been an industry standard for the cultivation of Escherichia coli as far back as the 1950s.These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins. This media has the addition of Kanamycin at 0.05gms/L for use with Kanamycin resistant strains and cells harbouring selection plasmids containing the Kanamycin resistance gene.Peptides and peptones are provided by tryptone. Vitamins and certain trace elements are provided by yeast extract. Sodium ions for transport and osmotic balance are provided by sodium chloride. Tryptone is used to provide essential amino acids to the growing bacteria, while the yeast extract is used to provide a plethora of organic compounds helpful for bacterial growth. -
Legionella Cystine Free Medium is intended for use in conjunction with Legionella CYE Medium (PP0200) as a secondary diagnostic medium for confirmation of a previously isolated organism. Although it contains Ferric Pyrophosphate and α-ketoglutarate it does not contain any L-Cysteine Hydrochloride. NB : This is a base medium only and will not sustain the growth of Legionella spp. -
This is a selective medium for the isolation of Legionella spp is used primarily in water and environmental laboratories. The base agar contains Yeast Extract as a source of protein, Charcoal to neutralise growth-inhibiting substances and is supplemented with Ferric Pyrophosphate as a source of iron, L-cysteine hydrochloride and α-ketoglutarate to form amino acid and chelate respectively. ACES buffer is incorporated to maintain the optimal pH for growth. The medium is made selective by the addition of Glycine, Vancomycin & Polymyxin B to inhibit the majority of gram positive and gram negative organisms and Cycloheximide is also included to inhibit yeasts and fungi. -
This is a selective medium for the isolation of Legionella spp used primarily in clinical and environmental laboratories. The base agar contains Yeast Extract as a source of protein, Charcoal to neutralise growth-inhibiting substances and is supplemented with Ferric Pyrophosphate as a source of iron, L-Cysteine hydrochloride and α-ketoglutarate to form amino acid and chelate respectively. ACES buffer and Potassium hydroxide are incorporated to maintain the optimal pH for growth. The medium is made selective by the addition of Cefamandole and Polymixin to inhibit most gram positive and gram negative organisms and Cyclohexamide is also included to inhibit yeasts and fungi. -
This is a basic medium for the cultivation of Legionella spp. It is intended primarily for use as a secondary diagnostic medium in conjunction with Legionella Cystine Free Medium (PP0201) for confirmation of a previously isolated organism. NB: This is a basal medium only and although it will sustain the growth of Legionella spp. It contains no selective supplements. It is therefore not recommended as a means of primary isolations from clinical samples. -
One of several media available as a selective identification medium for the isolation and identification of Listeria monocytogenes in food & clinical laboratories. Using Columbia Agar as the base Lithium Chloride is included to inhibit enterococci and Acriflavine to inhibit some other gram positive and gram negative organisms that may be present in such specimens. It is made further selective by the addition of the antimicrobials Cefoxitin, Colistin & Fosfomycin with Amphotericin included to inhibit any yeasts present. Aesculin is present as an indicator since Listeria monocytogenes will hydrolyse it and the associated reaction with the Ferric Ammonium Citrate gives rise to a black precipitate around the colonies. -
This is a selective medium for the isolation and differentiation of bile tolerant gram-negative (enteric) and gram-positive (staphylococci and enterococci) organisms and has uses in all areas of bacteriology. It has the disadvantage that many strains of Proteus spp will spread on it and for this reason MacConkey Agar without Salt may be preferred. -
This is a selective medium primarily for the isolation of Enterobacteriacae from waters & sewage. This media differs from the original MacConkey formulation in that as well as Bile Salts, Crystal Violet has been included as an additional selective agent. This has the effect of inhibiting gram-positive micrococci.
