Legionella BCYE with GVPC

Legionella BCYE with GVPC

This is a selective medium for the isolation of Legionella spp is used primarily in water and environmental laboratories. The base agar contains Yeast Extract as a source of protein, Charcoal to neutralise growth-inhibiting substances and is supplemented with Ferric Pyrophosphate as a source of iron, L-cysteine hydrochloride and α-ketoglutarate to form amino acid and chelate respectively. ACES buffer is incorporated to maintain the optimal pH for growth. The medium is made selective by the addition of Glycine, Vancomycin & Polymyxin B to inhibit the majority of gram positive and gram negative organisms and Cycloheximide is also included to inhibit yeasts and fungi.

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Organisms Ref. No Result
Legionella pneumophila NCTC 11192 White/grey translucent colonies
Legionella anisa NCTC 11974 White/grey translucent colonies
Escherichia coli NCTC 12241 Inhibited
Staphylococcus aureus NCTC 12981 Inhibited