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KM0038 Brilliant Green Agar is used for the selective isolation of Salmonella species from clinical specimens and food samples. Brilliant Green Agar was first cited by Kristensen et al. and later modified to improve selectivity. The medium is highly selective and may not be suitable for the isolation of Salmonella spp. from samples where numbers of salmonellae are low. If this is suspected to be the case, or if the sample is suspected to contain Salmonella typhi, other selective media such as Xylose Lysine Deoxycholate (XLD) Agar (E&O KM0013), Hektoen Enteric Agar (E&O KM0032) or Deoxycholate Citrate Agar (E&O KM0050) may be inoculated with the specimen in parallel. The United States Pharmacopeia indicates that the medium may be used to confirm the absence of salmonellae in nutritional and dietary supplements. The medium may also be used as a secondary plating medium for subculture from selective enrichment media during food and environmental testing, and as a primary isolation medium in the identification of Salmonella species from clinical specimens according to Public Health England’s UK Standards for Microbiology Investigations. -
A selective medium for the isolation and detection of dermatophytic fungi originally developed by Taplin et al. Dermatophytes appear as fluffy colonies with reddening of the medium while other fungi cause the medium to become yellow due to acid production. Yeasts will also grow on this medium but are readily distinguished by their distinct white/creamy colonies and distinctive smell. Soy peptone provides the required carbon, nitrogen and vitamins. Glucose is a fermentable carbohydrate. The inclusion of phenol red assists in the differentiation between saprophytic and environmental fungi. Although the low pH (pH 5.5) of the medium inhibits most bacteria chloramphenicol and cycloheximide are often added to further reduce the risk when processing material that may be more heavily contaminated. NB: Prolonged incubation should be avoided as this may cause fungi other than dermatophytes to turn the medium red. Related Supplements : LS0050 Chloramphenicol Selective Supplement
