KM0184 – Dermatophyte Test Medium

//KM0184 – Dermatophyte Test Medium

KM0184 – Dermatophyte Test Medium

A selective medium for the isolation and detection of dermatophytic fungi originally developed by Taplin et al.
Dermatophytes appear as fluffy colonies with reddening of the medium while other fungi cause the medium to become yellow due to acid production. Yeasts will also grow on this medium but are readily distinguished by their distinct white/creamy colonies and distinctive smell.
Soy peptone provides the required carbon, nitrogen and vitamins. Glucose is a fermentable carbohydrate. The inclusion of phenol red assists in the differentiation between saprophytic and environmental fungi. Although the low pH (pH 5.5) of the medium inhibits most bacteria chloramphenicol and cycloheximide are often added to further reduce the risk when processing material that may be more heavily contaminated.
NB: Prolonged incubation should be avoided as this may cause fungi other than dermatophytes to turn the medium red.

Related Supplements :
LS0050 Chloramphenicol Selective Supplement

SKU: KM0184 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Candida albicans NCPF 3179 Growth, yellow colonies
Trichophyton rubrum NCPF 419 Growth, white fluffy colonies with reddening of the medium
Apergillus brasiliensis NCPF 2275 Inhibited

 

Formula*

g/L
Soy peptone 10.5
Glucose 10.0
Phenol red 0.2
Agar 20.0
Total 40.7
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 40.7g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes with frequent stirring to completely dissolve the medium. Cool to 45-50°C and aseptically add 2 vials of DTM selective supplement LS7061, which consists of cycloheximide and chloramphenicol. Mix well and dispense into appropriate sterile containers.