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This is one of several selective media available for the isolation of Campylobacter spp. in clinical, food and environmental laboratories. Campylobacter agar base is based on the formulation from Bolton and Robertson. The peptone is the source of the required nitrogen, carbon and vitamins. Sodium chloride maintains the osmotic balance in the medium. The medium is enriched with lysed horse blood and made selective by the addition of cefoperazone, to suppress other enteric organisms, and amphotericin to suppress yeast and fungal growth (Preston supplement LS0010). Related Supplements : LS0009 Campylobacter (Skirrow) Selective Supplement, LS0010 Campylobacter (Preston) Selective Supplement, Lysed Blood -
Hektoen enteric agar was developed by King and Metzger as a differential selective medium for the isolation of Shigella spp. and Salmonella spp. species from enteric pathological samples. The meat peptone and yeast extract provide the required nitrogen, carbon and vitamins. Lactose, sucrose and salicin are fermentable carbohydrates. Bromothymol blue is added as a pH indicator in order to identify carbohydrate fermenting organisms. The combination of ferric ammonium citrate and sodium thiosulfate allows the production of hydrogen sulphide. Hydrogen sulphide positive colonies produce black centred colonies. Sodium chloride maintains the osmotic balance. The bile salts and acid fuchsin inhibit Gram-positive organisms.
