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Sterile Isotonic Saline suitable for use in preparation of food samples and/or as a rinse during examination of equipment etc. prior to culture. It can also be used as a general purpose diluent in many areas of the laboratory. -
This is a variation on sterile isotonic Saline which is suitable for use in preparation of food samples and/or as a rinse during examination of equipment etc. prior to culture. It can also be used as a general purpose diluent in many areas of the laboratory. The addition of glass beads will allow dense material to be broken down and aid the isolation of any bacteria that may be present in “clumps”. This particular product is double wrapped and terminally sterilised by Ethylene oxide. -
Universal Isotonic Saline Solution. -
A medium for the selective enrichment of Salmonella spp from both clinical and food samples. It is a buffered Lactose Peptone Broth to which Sodium Biselenite is added as the selective agent. Subcultures should be made from the top 1/3 of the broth after not more than 24 hours incubation as after this time there is a loss of selectivity. -
This medium can be used as a screening method for the differentiation of Enterobacteriaceae based on the ability of some species to utilise citrate as a sole source of carbon. It is often used as a screening test for Klebsiella pneumoniae (Positive reaction) while Escherichia coli is negative. Other uses included distinguishing between species of citrate positive Salmonellae (e.g. Salmonella enteritidis) and those that are negative (e.g. Salmonella typhi and Salmonella paratyphi A). The medium is inoculated by stabbing the organism (in pure culture) into the medium. A positive result produces a change of colour from green to bright blue and a negative reaction leaves the colour unchanged. -
This is an aqueous solution of Sodium Hydroxide (2%) with Phenol Red Indicator suitable for use in the digestion of Sputum samples prior to culture of the samples for Mycobacterium spp. It is generally used in conjunction with Sputum Neutralising Buffer (BM1324). -
This is an aqueous solution of Sodium Hydroxide (4%) suitable for use in the digestion of Sputum samples prior to culture. It is generally used in conjunction with Sputum Neutralising Buffer (BM1325). -
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This is a sterile aqueous solution of Potassium di-hydrogen phosphate (16.0 % w/v) with Phenol Red Indicator suitable for use in the neutralisation following the digestion of sputum with Sodium hydroxide prior to culture. It is generally used in conjunction with BM1322 Sodium hydroxide 4%. -
This is a sterile aqueous solution of Potassium Di-Hydrogen Ortho-phosphate (16.0 % w/v) suitable for use in the neutralisation following the digestion of Sputum with Sodium Hydroxide prior to culture. It is generally used in conjunction with Sodium Hydroxide 4% with Phenol Red Indicator 0.2%. -
This medium is utilised for the transportation and cryopreservation of Streptococcus pneumoniae & Neisseria meningitidis isolates. -
This is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp particularly Mycobacterium bovis. This medium is used primarily in the veterinarian sector. The medium is based on the original formulation of Lowenstein that was subsequently modified by Jensen, the medium contains pyruvate and egg which provide the required protein and fatty acids. It differs from Lowenstein-Jensen Medium in that Sodium Pyruvate has replaced the Glycerol, which has been demonstrated to be inhibitory to some species, particularly M.bovis. The coagulation of the egg albumin during preparation also provides a solid surface for inoculation purposes. Malachite green is incorporated into the medium to inhibit contaminating organisms other than the mycobacteria that may still be present in the specimen after decontamination. Lowenstein-Jensen slopes should be inoculated with pre-treated specimens and incubated at 35-37°C for 8 weeks in 5-10% CO2. Container caps should be left loose for the first week of incubation to allow for circulation of the carbon dioxide as this will help to stimulate growth. Caps should then be tightened to prevent any dehydration of the medium.
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This product may be used as an alternative pre-treatment solution to lessen the background flora of samples before the testing for Mycobacteria species. This reagent should only be used for samples that routinely produce contaminated cultures after processing with an alkaline digestant. -
This buffer is intended primarily for use as a neutralising agent following treatment with alkaline compounds during the decontamination and homogenisation process of Sputum specimens prior to inoculation onto appropriate culture media for the isolation of Mycobacterium spp. -
A nutritious broth medium formulated by Todd and Hewitt for the production of antigenic streptococcal haemolysin. The broth is also used to cultivate streptococci prior to serological grouping. Normally the use of fermentable sugars in the broth would inactivate the haemolysin due to acid production. This is prevented by the use of buffers to maintain the pH in this broth. -
This medium is based on the formula described by Kupferburg, Johnson and Sprince for the selective isolation of Trichomonas spp. The medium is selective due to the inclusion of the broad spectrum antibiotic, chloramphenicol, to inhibit a wide range of Gram-positive and Gram-negative bacterial species. This medium does not contain an antifungal agent and Candida spp. will not be suppressed. However, the growth of Candida spp. does not interfere with that of Trichomonas spp. The inclusion of methylene blue as a redox indicator allows for the visualisation of any significant oxygen diffusion in the medium. Cultures may be examined microscopically after 48 hours incubation at 37°C for the presence of flagellate protozoans. If a negative result is obtained then the culture may be re-incubated for a further 72 hours.
