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There are a number of media available for the selective isolation of Neisseria gonorrhoeae of which this is one. Based on the medium developed by Thayer & Martin, it includes Corn Starch to absorb toxic metabolites and is buffered to maintain a neutral pH. The medium is made selective by the inclusion of VCAT (Vancomycin, Colistin, Amphotericin B & Trimethoprim). Vancomycin and Colistin inhibit the growth of the majority of contaminating organisms likely to be present. Trimethoprim is included to reduce the spreading of Proteus spp and Amphotericin B to inhibit yeasts and fungi. Yeast Extract and Glucose are also added as further enrichment along with laked horse blood to ensure an more luxuriant growth of Neisseria gonorrhoeae. -
There are a number of media available for the selective isolation of Neisseria gonorrhoeae of which this is one. Based on the medium developed by Thayer & Martin, it includes Corn Starch to absorb toxic metabolites and is buffered to maintain a neutral pH. The medium is made selective by the inclusion of VCNT (Vancomycin, Colistin, Nystatin & Trimethoprim). Vancomycin and Colistin inhibit the growth of the majority of contaminating organisms likely to be present. Trimethoprim is included to reduce the spreading of Proteus spp and Nystatin to inhibit yeasts and fungi. Yeast Extract and Glucose are also added as further enrichment along with laked horse blood to ensure an more luxuriant growth of Neisseria gonorrhoeae. -
Helicobacter Pylori Medium with 10% Horse Serum, Cefsulodin (10mg/L), Vancomycin (10mg/L) & Amphoteracin (20mg/L) This is a selective medium for the isolation of Helicobacter pylori from clinical samples. The medium is based on a modification of Campylobacter CCDA Blood Free Medium with Charcoal, Ferrous Sulphate and Sodium Pyruvate replacing the Horse Blood and is made selective by the addition of Vancomycin and Cefsulodin to suppress other bacteria and Amphoteracin to inhibit yeasts. 10% Horse Serum is also added to promote optimum growth of helicobacter. -
A selective culture medium for the isolation and differentiation of Corynebacterium diphtheriae types, Hoyle’s medium allows for rapid growth of the organisms and normally 18 hours incubation should be sufficient for a diagnosis. As the medium is highly selective, inoculation should be by rubbing the swab (or other material) over the entire surface of the agar, there is no need to spread the inoculum with a loop indeed doing so can cause the organism to be missed especially when they are present only in small numbers. -
Iso-Sensitest with 5% Horse Blood & NAD (20mg/L) (25ml per dish) This is a defined medium suitable for antimicrobial susceptibility testing and on which most organisms will grow. The medium has been enriched with Horse Blood to meet the demands of the more fastidious organisms and NAD (Nicotinamide Adenine Dinucleotide) is also included to further enhance the growth of Haemophilus spp. This medium is included in the recommendations of BSAC as being appropriate for the susceptibility testing of Haemophilus spp. -
LB media formulations have been an industry standard for the cultivation of Escherichia coli as far back as the 1950s. These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins. This medium has the addition of carbenicillin at 100mg/L for use with transformed cells harbouring selection plasmids containing carbenicillin resistance genes. Peptides and essential amino acids are provided by tryptone. Vitamins and certain trace elements are provided by yeast extract and sodium chloride provides osmotic balance. -
LB media formulations have been an industry standard for the cultivation of Escherichia coli as far back as the 1950s.These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins. This media has the addition of Kanamycin at 0.05gms/L for use with Kanamycin resistant strains and cells harbouring selection plasmids containing the Kanamycin resistance gene.Peptides and peptones are provided by tryptone. Vitamins and certain trace elements are provided by yeast extract. Sodium ions for transport and osmotic balance are provided by sodium chloride. Tryptone is used to provide essential amino acids to the growing bacteria, while the yeast extract is used to provide a plethora of organic compounds helpful for bacterial growth. -
LB media formulations have been an industry standard for the cultivation of Escherichia coli as far back as the 1950s.These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins. This medium has the addition of kanamycin at 50mg/L and chloramphenicol at 34mg/L, for use with kanamycin and chloramphenicol resistant strains and cells harbouring selection plasmids containing the kanamycin and rifampicin resistance genes. Peptides, peptones and essential amino acids are provided by tryptone. Vitamins and certain trace elements are provided by yeast extract and sodium chloride provides osmotic balance.
