Columbia Agar with 7% Sheep Blood and CNA Supplement / Sabouraud Dextrose Agar with Chloramphenicol

///Columbia Agar with 7% Sheep Blood and CNA Supplement / Sabouraud Dextrose Agar with Chloramphenicol

Columbia Agar with 7% Sheep Blood and CNA Supplement / Sabouraud Dextrose Agar with Chloramphenicol

Side 1: Columbia Blood Agar with 7% Sheep Blood & CNA Supplement
This is a selective medium for the isolation of Staphylococcus and Streptococcus spp. Based on columbia agar, it is enriched by the addition of sheep blood (7%) the medium is also made selective by the inclusion of colistin and nalidixic acid to suppress the growth of the majority of Gram negative bacteria. The addition of sheep blood to the medium allows for good colonial appearance, pigment production and excellent haemolysis from beta-haemolytic streptococci.
Side 2: Sabouraud Dextrose Agar with Chloramphenicol
A selective medium for the isolation of yeasts and fungi. Sabouraud dextrose agar is suitable for use in all areas of Mycology. The low pH of the medium inhibits most bacteria, however in this formulation chloramphenicol (150mg/L) is added to further reduce the risk when processing material that may be heavily contaminated particularly with coliforms.

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Product Description

Columbia with 7% Sheep Blood & CNA

Organisms Ref. No Result
Streptococcus pyogenes NCTC 12696 Small white colonies with β-haemolysis
Streptococcus aureus NCTC 12981 White colonies
Escherichia coli NCTC 12241 Inhibited

 

Sab Dex with Chloramphenicol (150mg/L)

Organisms Ref. No Result
Candida albicans NCPF 3179 White colonies (Yeast smell)
Aspergillus brasiliensis NCPF 2275 White colonies. Pale yellow
underside with hyphae
Escherichia coli NCTC 12241 Inhibited

 

Recommended Incubation: For yeasts: Aerobically at 25 ± 1oC for 36-48 hours.
For fungi: Aerobically at 25 – 30oC for 5-10 days
Other organisms: Aerobically at 37 ± 1oC for 18 – 24 hours