KM0055 – Mycological Agar

//KM0055 – Mycological Agar

KM0055 – Mycological Agar

Mycological agar is a selective medium for the isolation of pathogenic fungi, particularly dermatophytes, from clinical specimens.
Enzymatic digest of soybean meal provides the required carbon, nitrogen and vitamins. Glucose is an energy source for the metabolism of fungi. The addition of chloramphenicol further reduces the risk of bacterial contamination when processing material that may be heavily contaminated with coliforms. Cycloheximide should also be added (0.4g/L) to suppress the growth of commensal yeasts and saprophytic fungi.

 

SKU: KM0055 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Candida albicans NCPF 3179 Growth, white colonies
Trichophyton rubrum NCPF 419 Growth, white mycelia with dark underside
Escherichia coli NCTC 12241 Inhibited
Aspergillus brasiliensis NCPF 2275 Inhibited

 

Formula*

g/L
Enzymatic digest of soybean meal 10.0
Glucose 10.0
Chloramphenicol 0.05
Agar 16.0
Total 36.0
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 36g of the medium in one litre of deionised / purified water. Add 0.4g of cycloheximide. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Sterilise at 121°C for 15 minutes. Cool to 45-50°C and aseptically dispense into appropriate sterile containers.