This is a selective enrichment broth for the isolation of Salmonella spp. primarily from food and food product samples and conforms to the requirements ISO 6579:2002. It can however be used in other areas including clinical and environmental specimens. Salmonella spp. reduce tetrathionate and will proliferate in the medium whilst most other enteric organisms are inhibited. Unlike the older traditional tetrathionate broth the addition of novobiocin (40 mg/l) improves the inhibition of Proteus spp.
Immediately prior to use it is necessary to add 20 ml/l of 2% iodine/iodide solution (BM0946). Once the iodine/iodide solution has been added the medium should be used immediately and cannot be stored for future use.
NB: As this is an opaque medium, the turbidity of the broth alone cannot be used as an indication of growth.
Related Supplements :
LS0024 Novobiocin Supplement (20mgs/L)