KM0063 -Muller-Kauffmann Tetrathionate Broth (MKTTn)

//KM0063 -Muller-Kauffmann Tetrathionate Broth (MKTTn)

KM0063 -Muller-Kauffmann Tetrathionate Broth (MKTTn)

This is a selective enrichment broth for the isolation of Salmonella spp. primarily from food and food product samples and conforms to the requirements ISO 6579:2002. It can however be used in other areas including clinical and environmental specimens. Salmonella spp. reduce tetrathionate and will proliferate in the medium whilst most other enteric organisms are inhibited. Unlike the older traditional tetrathionate broth the addition of novobiocin (40 mg/l) improves the inhibition of Proteus spp.
Immediately prior to use it is necessary to add 20 ml/l of 2% iodine/iodide solution (BM0946). Once the iodine/iodide solution has been added the medium should be used immediately and cannot be stored for future use.
NB: As this is an opaque medium, the turbidity of the broth alone cannot be used as an indication of growth.

Related Supplements :
LS0024 Novobiocin Supplement (20mgs/L)

SKU: KM0063 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Salmonella typhimurium NCTC 12203 Growth
Salmonella enteritidis NCTC 12694 Growth
Escherichia coli NCTC 12241 Inhibited
Enterocococcus faecalis NCTC 12697 Inhibited

 

Formula*

g/L
Meat extract 4.3
Enzymatic digest of casein 8.6
Sodium chloride 2.6
Calcium carbonate 38.7
Sodium thiosulphate (anhydrous)** 30.5
Ox bile 4.78
Brilliant green 0.0096
Total 89.5
*Adjusted/supplemented as required to meet performance requirements
**Equivalent to 47.8g of sodium thiosulphate pentahydrate

Preparation

Suspend 89.5g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise by boiling for 1 minute with frequent stirring to completely dissolve the medium. Cool to 45-50°C and aseptically add 2 vials of selective supplement novobiocin (LS0024). Mix well and dispense into appropriate sterile containers. Prior to use add 20 ml/l of 2% iodine/iodide solution (BM0946).