KM0067 – Violet Red Bile Glucose Agar

//KM0067 – Violet Red Bile Glucose Agar

KM0067 – Violet Red Bile Glucose Agar

Violet Red Bile Glucose Agar (VRBGA) is a selective medium for the isolation and enumeration of Enterobacteriaceae in food products. It is a modification of the original Violet Red Bile Agar with the lactose being replaced with glucose. As all Enterobacteriaceae ferment glucose VRBGA allows for a wider range of organisms to be detected. This medium conforms to the requirements of the Harmonised USP/EP/JP.
Yeast extract and pancreatic digest of gelatin provide the required carbon, nitrogen and vitamins. Glucose is a fermentable carbohydrate and neutral red is a pH indicator. Sodium chloride maintains the osmotic balance. The medium is made selective by the inclusion of bile salts and crystal violet to inhibit Gram-positive and non-enteric organisms.

SKU: KM0067 Category:

Additional Information

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Product Description

Organisms Ref. No Result
Escherichia coli ATCC 8739 Growth, red/purple colonies with or without precipitation halo
Salmonella typhimurium ATCC 14028 Growth, red/purple colonies with or without precipitation halo
Enterococcus faecalis ATCC 29212 Inhibited

 

Formula*

g/L
Yeast extract 3.0
Pancreatic digest of gelatin 7.0
Sodium chloride 5.0
Bile salts 1.5
Glucose 10.0
Neutral red 0.03
Crystal violet 0.002
Agar 12.0
Total 38.5
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 38.5g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Cool to 45-50°C and aseptically dispense into appropriate sterile containers.