Violet Red Bile Glucose Agar

//Violet Red Bile Glucose Agar

Violet Red Bile Glucose Agar

SKU: KM0067 Category:

Violet Red Bile Glucose Agar (VRBGA) is a selective medium for the isolation and enumeration of Enterobacteriaceae in food products. It is a modification of the original Violet Red Bile Agar with the lactose being replaced with glucose. As all Enterobacteriaceae ferment glucose VRBGA allows for a wider range of organisms to be detected. This medium conforms to the requirements of the Harmonised USP/EP/JP.
Yeast extract and pancreatic digest of gelatin provide the required carbon, nitrogen and vitamins. Glucose is a fermentable carbohydrate and neutral red is a pH indicator. Sodium chloride maintains the osmotic balance. The medium is made selective by the inclusion of bile salts and crystal violet to inhibit Gram-positive and non-enteric organisms.

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Prepared Medium Appearance

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Product Description

Organisms Ref. No Result
Escherichia coli ATCC 8739 Growth, red/purple colonies with or without precipitation halo
Salmonella typhimurium ATCC 14028 Growth, red/purple colonies with or without precipitation halo
Enterococcus faecalis ATCC 29212 Inhibited



Yeast extract 3.0
Pancreatic digest of gelatin 7.0
Sodium chloride 5.0
Bile salts 1.5
Glucose 10.0
Neutral red 0.03
Crystal violet 0.002
Agar 12.0
Total 38.5
*Adjusted/supplemented as required to meet performance requirements


Suspend 38.5g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Cool to 45-50°C and aseptically dispense into appropriate sterile containers.