KM0093 – Brucella Medium Base

//KM0093 – Brucella Medium Base

KM0093 – Brucella Medium Base

Brucella Medium Base is a general purpose medium for the cultivation of Brucella spp. and other fastidious microorganisms. Brucella Medium Base has been developed from the APHA formulation for Albimi broth.
The peptones act as carbon, nitrogen and vitamin source in this medium. Glucose is a fermentable carbohydrate and sodium chloride maintains the osmotic balance of the medium.

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

OrganismRef. No.Result
Brucella ovisATCC 25840Growth, white colonies
Escherichia coliATCC 25922Growth, cream colonies

Formula*

FORMULA* g/L
Peptones22
Sodium chloride 5
Glucose 1
Sodium bisulfite 0.1
Agar 14.9
Total 43

Preparation

Suspend 43.0g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes. Once cooled any required nutritional or selective supplements may be added. Aseptically dispense into appropriate sterile containers.