Brucella Medium Base is a general purpose medium for the cultivation of Brucella spp. and other fastidious microorganisms. Brucella Medium Base has been developed from the APHA formulation for Albimi broth.
The peptones act as carbon, nitrogen and vitamin source in this medium. Glucose is a fermentable carbohydrate and sodium chloride maintains the osmotic balance of the medium.
Brucella Medium Base
Additional Information
| Container | |
|---|---|
| Shelf Life (days) | |
| Storage Temp (°C) | |
| Dehydrated Medium Appearance | |
| Prepared Medium Appearance | |
| Dehydrated Medium Colour | |
| Prepared Medium Colour | |
| Dehydrated PH | |
| Prepared PH | |
| Recommended Incubation | For Brucella spp. – Incubate under CO2 at 37 ± 1°C for 18 – 96 hours. |
Product Description
| Organism | Ref. No. | Result |
|---|---|---|
| Brucella ovis | ATCC 25840 | Growth, white colonies |
| Escherichia coli | ATCC 25922 | Growth, cream colonies |
Formula*
| FORMULA* | g/L |
|---|---|
| Peptones | 22 |
| Sodium chloride | 5 |
| Glucose | 1 |
| Sodium bisulfite | 0.1 |
| Agar | 14.9 |
| Total | 43 |
Preparation
Suspend 43.0g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes. Once cooled any required nutritional or selective supplements may be added. Aseptically dispense into appropriate sterile containers.