Kligler Iron Agar

//Kligler Iron Agar

Kligler Iron Agar

SKU: KM0111 Category:

Kligler iron agar is used to differentiate between some of the enterobacteriacae on the basis of three reactions: fermentation of lactose and glucose and the production of hydrogen sulphide.
Kligler iron agar is a modification of the original formulation developed by Kligler.
It incorporates the principles of Russell’s double sugar agar with Kligler ‘s lead acetate agar.
The peptone provides the required nitrogen, carbon and vitamins. Lactose and glucose are carbohydrates.
Acid production from their fermentation is detected by the phenol red pH indictor. Sodium thiosulphate is reduced to hydrogen sulphide which is detected by the ferric citrate indicator.
Sodium chloride maintains the osmotic balance.

Additional Information


Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Slant Butt Gas H2S
Escherichia coli NCTC 12241 Acid Acid +
Salmonella typhimurium NCTC 12023 Alkaline Acid + +
Pseudomonas aeruginosa NCTC 12903 Alkaline Alkaline
Shigella flexneri ATCC 12022 Alkaline Acid
Proteus mirabilis ATCC 29906 Alkaline Acid +
Citrobacter freundii ATCC 43864 Acid Acid + +



Peptone 20.0
Sodium chloride 5.0
Lactose 10.0
Glucose 1.0
Ferric citrate 0.5
Sodium thiosulphate 0.3
Phenol red 0.025
Agar 12.0
Total 49.0
*Adjusted/supplemented as required to meet performance requirements


Suspend 49g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes with frequent stirring to completely dissolve the medium. Cool to 45-50°C and dispense into appropriate sterile containers.