KM0114 – Luria Bertani Agar (Miller)

//KM0114 – Luria Bertani Agar (Miller)

KM0114 – Luria Bertani Agar (Miller)

Luria Bertani (LB) agar (Miller) is used for the cultivation and maintenance of recombinant strains of Escherichia coli and is based on the formulation stated by Miller. This medium is typically used in molecular biology procedures such as the detection of phage or plasmid transformed bacteria. LB agar (Miller) is prepared using 10 g/L of sodium chloride and this level varies from that described by Lennox.
Enzymatic digest of casein provides the required nitrogen and carbon for organism growth. Vitamin B complex required by recombinant strains of E. coli are supplied by the yeast extract. Sodium chloride maintains the osmotic balance and provides sodium ions for membrane transport.

Related Supplements :
LS0035 Ampicillin Selective Supplement, LS0037 Kanamycin Selective Supplement

SKU: KM0114 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Escherichia coli NCTC 12241 Growth, cream colonies

 

Formula*

g/L
Enzymatic digest of casein 10.0
Yeast extract 5.0
Sodium chloride 10.0
Agar 15.0
Total 40.0
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 40g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Sterilise at 121°C for 15 minutes. Cool to 45-50°C, add any required antibiotic supplements, mix well and dispense into appropriate sterile containers.