Letheen Broth Modified

//Letheen Broth Modified

Letheen Broth Modified

Letheen broth modified is intended for use in the isolation of microorganisms from cosmetics.
The enzymatic digest of animal tissue, enzymatic digest of casein, yeast extract and beef extract act as carbon, nitrogen and vitamin sources in this medium. Sodium chloride maintains the osmotic balance of the medium. The sodium bisulfite and lecithin inactivates quaternary ammonium compounds. Polysorbate 80 must be added to the medium prior to sterilisation. Polysorbate 80 neutralises phenols, formalin, hexachlorophene, and in combination with the lecithin, ethanol.

SKU: KM0120 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

OrganismRef. No.Result
Escherichia coliATCC 8739Growth, cream colonies
Enterococcus faecalisNCTC12697Growth, white colonies
Pseudomonas aeruginosaATCC 9027Growth, cream colonies
Staphylococcus aureusATCC 6538Growth, white colonies

Formula*

FORMULA* g/L
Enzymatic digest of animal tissue 20
Enzymatic digest of casein 5
Yeast extract 2
Sodium chloride5
Beef extract 5
Lecithin 0.7
Sodium bisulfite0.1
Total 37.8

Preparation

Suspend 37.8g of the medium in one litre of deionised / purified water. Add 5g of polysorbate 80 then allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes. Cool to 45-50°C and aseptically dispense into appropriate sterile containers.