Nutrient Broth

Nutrient Broth

SKU: KM0142 Category: Tag:

A general-purpose medium for the cultivation of organisms that are less fastidious in their nutritional requirements.
The beef extract, peptone and yeast extract act as carbon, nitrogen and vitamin sources in this medium. Sodium chloride maintains osmotic balance.


Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Escherichia coli NCTC 12241 Growth
Staphylococcus aureus NCTC 12981 Growth



Beef extract 1.0
Peptone 5.0
Yeast extract 2.0
Sodium chloride 5.0
Total 13.0
*Adjusted/supplemented to meet performance requirements


Suspend 13g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Sterilise at 121°C for 15 minutes.