Selenite cystine broth is a modification of selenite F broth and is for the selective enrichment of Salmonellae spp. from clinical, food and environmental specimens.
The peptone acts as a nitrogen, carbon and vitamin source. Lactose is a fermentable carbohydrate and sodium phosphate is a buffer. L-cystine is used to enhance the recovery of Salmonellae spp. in low numbers. The medium is made selective by the addition of sodium biselenite (KM8021).
Following overnight incubation subculture(s) are usually made on to one or more of the many selective enteric solid media.