KM0187 – Primary Membrane Lactose Glucuronide Agar (mLGA)

//KM0187 – Primary Membrane Lactose Glucuronide Agar (mLGA)

KM0187 – Primary Membrane Lactose Glucuronide Agar (mLGA)

Primary Membrane Lactose Glucuronide Agar (mLGA) is used for the differentiation and enumeration of Escherichia coli and other coliforms through a single membrane filtration technique.
The peptone and yeast extract provide the required nitrogen, carbon and vitamins. Sodium pyruvate protects injured cells enhancing recovery and growth of coliforms. Sodium lauryl sulphate is a selective agent that inhibits Gram-positive organisms. Lactose is a fermentable carbohydrate and phenol red is a pH indicator. Lactose fermentation will result in yellow colonies. X-glucuronide is a chromogenic substrate which can be cleaved by the enzyme ß-glucuronidase present in E. coli. This results in a blue colony, but in combination with lactose fermentations colonies will appear green.
NB: Prepared plates stored at 2–8°C may show formation of surface crystals which will disappear when plates are warmed to >20°C

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SKU: KM0187 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Escherichia coli NCTC 12241 Growth, green colonies
Citrobacter freundii ATCC 43864 Growth, yellow colonies
Salmonella typhimurium NCTC 12023 Growth, pink/red colonies
Enterococcus faecalis NCTC 12697 Inhibited

 

Formula*

g/L
Peptone 40.0
Yeast extract 6.0
Lactose 30.0
Phenol red 0.2
Sodium lauryl sulphate 1.0
Sodium pyruvate 0.5
X-Glucuronide 0.2
Agar 10.0
Total 88.0
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 88g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 115°C for 10 minutes with frequent stirring to completely dissolve the medium. Cool to 45-50°C and dispense into appropriate sterile containers.