LS0022 – Clostridium difficile Selective Supplement

//LS0022 – Clostridium difficile Selective Supplement

LS0022 – Clostridium difficile Selective Supplement

Clostridium difficile Selective Supplement
E&O Laboratories Ltd Clostridium Difficile Selective Supplement (LS0022) is an antibiotic supplement used to enhance the isolation of Clostridium difficile from faecal specimens.

 

SKU: LS0022 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Dehydrated Medium Colour

Solubility Description

Product Description

Organisms Ref. No Result
Clostridium difficile NCTC 11204 Growth
Escherichia coli NCTC 12241 Inhibited

 

Formula

Each vial contains:

Cefoxitin 4mg
D-cycloserine 125mg

Procedure

To reconstitute each vial aseptically add 2ml of Sterile Distilled Water. Close the vial and gently agitate to assist reconstitution. Avoid frothing and the resulting solution should be clear and free from visible particulate matter. Add the contents of the vial to 500ml of sterile base medium of choice prepared according to the manufacturer’s instructions and allowed to cool to 45°- 50°C.
Mix gently but thoroughly and pour aseptically into sterile Petri dishes.
Overnight storage at 2°-8°C is recommended to allow equilibration of the supplement within the medium. For extended storage plates should be contained in sealed plastic sleeves or similar packing.