LS5002 – Fraser Broth Selective Supplement

//LS5002 – Fraser Broth Selective Supplement

LS5002 – Fraser Broth Selective Supplement

Fraser Broth Selective Supplement
E&O Laboratories Ltd Fraser Broth Selective Supplement (LS5002) is an antibiotic supplement used to enhance the selective isolation of Listeria spp. in combination with Fraser Broth.

 

SKU: LS5002 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Dehydrated Medium Colour

PH

Solubility Description

Product Description

Organisms Ref. No Result
Listeria monocytogenes NCTC 11994 Growth & Black colonies
Escherichia coli NCTC 12241 Inhibited
Enterococcus faecalis NCTC 12697 Inhibited

 

Formula

Vial Contents per Vial per Litre
Ferric ammonium citrate 0.25g 0.5g
Nalidixic acid 10.0mg 20.0mg
Acriflavine hydrochloride 12.5mg 25.0mg

Procedure

Suspend the recommended manufacturer’s amount of dehydrated media in 500ml of distilled water and mix well to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 45 – 50°C, and aseptically add the contents of one vial of Fraser Broth Selective Supplement (LS5002).To reconstitute vial add 2 mls of distilled water and mix before addition to dehydrated media.