MacConkey Broth (Double Strength) with Cefotaxime (2mg/L)

MacConkey Broth (Double Strength) with Cefotaxime (2mg/L)

This is a selective medium for the isolation of ESBL (Extended Spectrum Beta-Lactamase) producing strains of Escherichia coli.
It should be noted that AmpC isolates may also be detected on this medium whilst non – ESBL organisms will be inhibited on this medium.
The inclusion of bromocresol purple indicator displays the acid production due to the lactose fermentation by means of a colour change from purple to yellow.
N.B. – This is double strength broth.

Additional Information



Shelf Life (days)

Storage Temp (°C)





Product Description

Organisms Ref. No Result
Escherichia coli (ESBL) NCTC 13441 Growth (Yellow colour in broth)
Proteus mirabilis NCTC 10975 Inhibited
Escherichia coli NCTC 12241 Inhibited
Staphylococcus aureus NCTC 12981 Inhibited


Recommended incubation: Incubate aerobically at 37 ± 1oC or 44 ± 1oC (depending on methodology) for 24-48 hours.