Mueller Hinton Agar is recommended for use in the antibiotic disk diffusion method by both the European Committee on Antibiotic Sensitivity Testing (EUCAST) and the Clinical Laboratory Standards Institute (CLSI). The medium contains low levels of divalent metal cations, such as calcium and magnesium, to minimise any interference with certain antibiotic classes e.g. aminoglycosides. Starch is also present to absorb any toxic metabolites that may be formed during growth. The medium is low in thymine & thymidine content and is therefore suitable for use in testing sulphonamides and trimethoprim without the addition of blood.
Mueller Hinton Agar
|Shelf Life (days)|
|Storage Temp (°C)|
|Escherichia coli||NCTC 12241||White /grey colonies|
|Staphylococcus aureus||NCTC 12981||Cream / yellow colonies|
Recommended incubation: Incubate aerobically at 35 ± 1°C for 16-20 hours.
Antibiotic sensitivity quality control testing performed using Escherichia coli (NCTC 12241), Staphylococcus aureus (NCTC 12973), Pseudomonas aeruginosa (NCTC 12903) and Enterococcus faecalis (NCTC 12697) according to EUCAST methodology.