Pasteurella Agar Base

//Pasteurella Agar Base

Pasteurella Agar Base

SKU: KM0204 Category:

Pasteurella Agar Base is an non-selective medium capable of supporting the growth of Pasteurella spp. Pasteurella spp.are spherical, ovoid or rod-shaped cells 0.3-1.0μm in diameter and 1.0-2.0μm in length. Cells are Gram negative, and occur singly, or in pairs or short chains. Bipolar staining may be seen and capsules may be present. All species are non-motile, and are facultatively anaerobic.(1)

With further enrichment using 5-10% blood and selective antimicrobials, most Pasteurella spp. can be isolated. There is no haemolysis on blood agar for Pasteurella spp. and the organism will not regularly exhibit satellitism around colonies of Staphylococcus spp. The peptones act as carbon, nitrogen and vitamin sources in this medium. Sodium chloride maintains the osmotic balance and the agar is a solidifying agent. The medium is made selective through the addition of LS0057, Pasteurella agar selective supplement.

(1) Standards Unit, Microbiology Services, PHE. 1995. UK Standards for Microbiology Investigations. Identification of Pasteurella species and Morphologically Similar Organisms. 3, 1-28.

Additional Information

Shelf Life (days)

Dehydrated Medium Appearance

Dehydrated Medium Colour

Dehydrated PH

Prepared Medium Appearance

Prepared Medium Colour

Prepared PH


Product Description

Organisms Ref. No Result
Pasteurella multocida NCTC 10322 Growth, grey colonies
Streptoccocus pneumoniae NCTC 12977 Inhibited

Recommended incubation: Incubate in 5-10% CO2 at 35-37°C for 18-48 hours


Peptones 20.0
Sodium chloride 5.0
Agar 15
*Adjusted/supplemented as required to meet performance requirements


Suspend 40.0g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes to completely dissolve the medium then cool to 45-50°C. Aseptically add the appropriate volume of sterile defibrinated sheep blood and add 2 vials of LS0057 Pasteurella agar selective supplement. Mix well and dispense into appropriate sterile containers.