Primary UTI / Columbia Agar with 7% DHB & CNA

///Primary UTI / Columbia Agar with 7% DHB & CNA

Primary UTI / Columbia Agar with 7% DHB & CNA

Side 1: Primary UTI Chromogenic Agar
This is a chromogenic medium based on CLED that has been developed to allow differentiation and presumptive identification of organisms typically found in urinary tract infections. Reduced electrolyte concentration prevents swarming of Proteus spp. A sophisticated binary chromogenic system and supplementation with tryptophan allows differentiation of enterococci (turquoise colonies), Proteus spp (clear colonies with a brown halo), Enterobacter spp (metallic blue colonies), staphylococci (white colonies), and E. coli (purple colonies).

Side 2: Columbia Agar w 7% Defibrinated Horse Blood & CNA
This is a selective medium for the isolation of Staphylococcus/ spp and Streptococcus spp. It is based on Columbia Agar enriched with defibrinated horse blood which promotes good colony appearance, pigment production and excellent haemolysis from beta-haemolytic streptococci. The medium is made selective by the inclusion of colistin and nalidixic acid to suppress growth of the majority of Gram-negative bacteria.

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Side 1

Organisms Ref. No Result
Escherichia coli NCTC 12241 Purple colonies
Staphylococcus aureus NCTC 12981 White colonies
Proteus mirabilis NTCC 10975 Clear colonies with Brown halo
Enterococcus faecalis NCTC 12697 Blue colonies
Klebsiella aerogenes NCTC 10006 Metallic blue colonies


Side 2

Organisms Ref. No Result
Staphylococcus aureus NCTC 12981 White colonies
Staphylococcus pyogenes NCTC 12696 White colonies with β-haemolysis
Streptococcus pneumoniae NTCC 12977 Grey/green colonies with α-haemolysis
Proteus mirabilis NCTC 10975 Inhibited
Pseudomonas aeruginosa NCTC 12903 Inhibited

Recommended Incubation: Incubate at 37°C ± 1°C in O2 for 18-24 hrs
Incubate S. pneumoniae at 37ºC ± 1ºC in 5% CO2 for 18-24 hours