Rappaport-Vassiliadis (MSRV) Medium Semi-Solid

//Rappaport-Vassiliadis (MSRV) Medium Semi-Solid

Rappaport-Vassiliadis (MSRV) Medium Semi-Solid

SKU: KM0150 Category:

Rappaport-Vassiliadis (MSRV) Medium Semi-Solid is a modification of Rappaport-Vassiliadis Soy Broth for detecting motile Salmonella spp. in faeces and food products.[1]
The original research on MSRV Medium revealed a semi-solid could be used as a rapid and sensitive test for isolating motile Salmonella spp. from food products following pre-enrichment or selective enrichment.[2]

The semi-solid medium allows motility to be detected as halos of turbid growth around the original point of inoculation.
The peptones are to provide vitamins, nutrients and nitrogen to encourage growth of Salmonella spp.
The salt maintains the osmotic balance and potassium dihydrogen phosphate is a buffer for stabilising the pH of the medium.
Malachite green is included as a selective agent that inhibits Gram-positive organisms and some Gram-negative organisms.

(1) ISO 6579-1:2017. Microbiology of the food chain – Horizontal method for the detection, enumeration and serotyping of Salmonella – Part 1: Detection of Salmonella spp.
(2) De Smedt J.M., Bolderdikj R., Rappold H. and Lautenschlaeger D. 1986. Rapid Salmonella Detection in Foods by Motility Enrichment on a Modified Semi-Solid Rappaport-Vassiliadis Medium. J. Food Prot. 49:510-514

Additional Information

Shelf Life (days)

Dehydrated Medium Appearance

Dehydrated Medium Colour

Dehydrated PH

Prepared Medium Appearance

Prepared Medium Colour

Prepared PH


Product Description

Organisms Ref. No Result
Salmonella typhimurium NCTC 12023 Growth, turbid zone extending from inoculation point
Salmonella enteritidis NCTC 12694 Growth, turbid zone extending from inoculation point
Escherichia coli NCTC 12241 Inhibited, possible growth without turbid zone
Enterococcus faecalis NCTC 12697 Inhibited


Recommended incubation: Aerobically at 41.5 ± 1°C for 24 ± 3 hours. Incubation for a further 24 hours can be carried out if negative results are seen on the plates.


Enzymatic digest of animal and plant tissue 4.6
Acid hydrolysate of casein 4.6
Sodium chloride 7.3
Potassium dihydrogen phosphate 1.5
Magnesium chloride anhydrous 10.9
Malachite green oxalate 0.04
Agar 2.7
Total 31.64
*Adjusted/supplemented as required to meet performance requirements



Suspend 14.238g of the medium in 450ml of deionised / purified water.
Allow the medium to soak whilst mixing for 10 minutes.
Heat with frequent agitation and boil for one minute to completely dissolve the medium.
DO NOT AUTOCLAVE this medium.
Cool to 45-50°C and aseptically add 1 vial of LS0160 MSRV Novobiocin Selective Supplement (or 2ml of BM4032 MSRV Novobiocin Solution) per 450ml of product. Allow to mix thoroughly then aseptically dispense into appropriate sterile containers.