In vitro diagnostic. Dehydrated KM0179 Yersinia Selective Agar Base powder can be reconstituted to form a selective plated medium for the isolation and enumeration of Yersinia species from clinical and food samples.
Yersinia Selective Agar
Additional Information
| Container | |
|---|---|
| Shelf Life (days) | |
| Storage Temp (°C) | |
| Dehydrated Medium Appearance | |
| Dehydrated Medium Colour | |
| Prepared Medium Colour | |
| Prepared Medium Appearance | |
| Dehydrated PH | |
| Prepared PH | |
| Recommended Incubation |
Product Description
| Organisms | Ref. No | Result |
| Y. enterocolitica | NCTC 12982 | Growth: clear colonies with red centre |
| Escherichia coli | NCTC 12241 | Inhibited |
| Staphylococcus aureus | NCTC 12981 | Inhibited |
Recommended incubation: Aerobically at 30 ± 1°C for 21 ± 3 hours.
Formula*
| g/L | |
| Peptones | 22.0 |
| Mannitol | 20.0 |
| Sodium pyruvate | 2.0 |
| Sodium chloride | 1.0 |
| Magnesium sulphate | 0.4 |
| Sodium deoxycholate | 0.01 |
| Neutral red | 0.03 |
| Crystal violet | 0.001 |
| Agar | 12.5 |
| Total | 58.041 |
| *Adjusted/supplemented as required to meet performance requirements |
Preparation
Suspend 58.0g/L of dried culture media in deionised/purified water. Soak with frequent agitation whilst mixing for 10 minutes to
completely dissolve dried culture medium.
Sterilise at 121°C for 15 minutes before cooling to 45-50°C.
Aseptically add the appropriate volume of LS1053 Yersinia (CIN) Selective Supplement.
Gently mix the final medium before aseptically dispensing the specified volume into appropriate sterile containers and allowing to
cool.