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This is a selective enrichment broth for the isolation of Salmonella spp. from pharmaceutical, food, dairy and environmental samples. Malachite Green and Magnesium Chloride are included in the formulation as selective agents due to their ability to inhibit most enteric organisms whilst allowing Salmonella spp. to multiply freely. Gram +ve bacteria and most other enteric bacteria, are typically susceptible to or inhibited by Malachite Green, the high osmotic pressure and/or the low pH of the medium. It should be noted that S.typhi and S.choleraesuis are sensitive to Malachite Green and may therefore be inhibited. This medium conforms to the requirements of the Harmonised USP/EP/JP. -
Sheep Blood Agar Base has been developed to provide a nutrient rich medium compatible with sheep blood for the cultivation of many bacteria, especially fastidious Streptococcus spp., without affecting haemolytic reactions. Alternative culture mediums, such as Blood agar base No. 2, can results in mixed haemolytic reactions for some Streptococcus spp. This is thought to occur due to the trace amounts of fermentable carbohydrates in yeast extract and the physiological difference between sheep and horse blood. The tryptone, peptone, and yeast extract provide the required nitrogen, carbon and vitamins in the medium. Sodium chloride maintains the osmotic balance. Related Supplements : LS0008 Staph/Strep Selective Supplement -
Violet Red Bile Glucose Agar (VRBGA) is a selective medium for the isolation and enumeration of Enterobacteriaceae in food products. It is a modification of the original Violet Red Bile Agar with the lactose being replaced with glucose. As all Enterobacteriaceae ferment glucose VRBGA allows for a wider range of organisms to be detected. This medium conforms to the requirements of the Harmonised USP/EP/JP. Yeast extract and pancreatic digest of gelatin provide the required carbon, nitrogen and vitamins. Glucose is a fermentable carbohydrate and neutral red is a pH indicator. Sodium chloride maintains the osmotic balance. The medium is made selective by the inclusion of bile salts and crystal violet to inhibit Gram-positive and non-enteric organisms.
