KM0189 – Sheep Blood Agar Base

//KM0189 – Sheep Blood Agar Base

KM0189 – Sheep Blood Agar Base

Sheep Blood Agar Base has been developed to provide a nutrient rich medium compatible with sheep blood for the cultivation of many bacteria, especially fastidious Streptococcus spp., without affecting haemolytic reactions.
Alternative culture mediums, such as Blood agar base No. 2, can results in mixed haemolytic reactions for some Streptococcus spp. This is thought to occur due to the trace amounts of fermentable carbohydrates in yeast extract and the physiological difference between sheep and horse blood.
The tryptone, peptone, and yeast extract provide the required nitrogen, carbon and vitamins in the medium. Sodium chloride maintains the osmotic balance.

Related Supplements :
LS0008 Staph/Strep Selective Supplement

SKU: KM0189 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Streptococcus pyogenes NCTC 12696 Growth, white colonies with β-haemolysis
Streptococcus pneumoniae ATCC 6305 Growth, grey/green colonies with α-haemolysis
Staphylococcus aureus NCTC 12981 Growth, white colonies
Escherichia coli NCTC 12241 Growth, opaque/grey colonies

 

Formula*

g/L
Tryptone 14.0
Peptone 4.5
Yeast extract 4.5
Sodium chloride 5.0
Agar 12.0
Total 40.0
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 40g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Sterilise at 121°C for 15 minutes. Cool to 45-50°C. Once cooled add 5 – 10% of defibrinated sheep blood and mix well. Aseptically dispense into appropriate sterile containers.