Sheep Blood Agar Base has been developed to provide a nutrient rich medium compatible with sheep blood for the cultivation of many bacteria, especially fastidious Streptococcus spp., without affecting haemolytic reactions.
Alternative culture mediums, such as Blood agar base No. 2, can results in mixed haemolytic reactions for some Streptococcus spp. This is thought to occur due to the trace amounts of fermentable carbohydrates in yeast extract and the physiological difference between sheep and horse blood.
The tryptone, peptone, and yeast extract provide the required nitrogen, carbon and vitamins in the medium. Sodium chloride maintains the osmotic balance.
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