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This buffer is intended primarily for use as a neutralising agent following treatment with alkaline compounds during the decontamination and homogenisation process of Sputum specimens prior to inoculation onto appropriate culture media for the isolation of Mycobacterium spp. -
This medium is recommended for the detection and enumeration of yeasts and moulds in food and dairy products. It can also be used for the cultivation of fungi although with the prolonged incubation necessary cultures may become overgrown by bacteria. The low pH (5.6) suppresses the growth of most bacteria and the low mineral content ensures good pigment production by fungi where appropriate. -
An osmotically controlled solution for the preparation of suspensions of food samples and for use as a diluent in dilution techniques for bacterial enumeration. The solution may also be used in the sampling of food production apparatus by the rinse and swab method. -
An osmotically controlled solution for the preparation of suspensions of food samples and for use as a diluent in dilution techniques for bacterial enumeration. The solution may also be used in the sampling of food production apparatus by the rinse and swab method. -
This is a variation on the common osmotically controlled Ringers solution, where glass beads have been added to enhance macerations in the preparation of suspensions of samples and for use as a diluent in dilution techniques for bacterial enumeration. The solution may also be used in the sampling of food production apparatus by the rinse and swab method. -
A long established selective medium for the isolation of yeasts and fungi suitable for use in all areas of Mycology. The low pH (5.6) of the medium inhibits most bacteria and spore structures and pigmentation of fungi are generally well developed on this medium. -
This is a selective medium for the isolation of yeasts and fungi and is particularly suitable for use in sterility testing. The low pH (5.6) of the medium is inhibitory to most bacteria and many diagnostic features such as spores and pigmentation are well developed on this medium. The formulation of this medium conforms to the requirements of the Harmonized USP/EP/JP. -
Sterile Saline (0.45%) is suitable for use in preparation of food samples and/or as a rinse fluid during examination of equipment etc. prior to culture. It can also be used as a general purpose diluent in many areas of the laboratory. -
Sterile Isotonic Saline suitable for use in preparation of food samples and/or as a rinse during examination of equipment etc. prior to culture. It can also be used as a general purpose diluent in many areas of the laboratory. -
This is a variation on sterile isotonic Saline which is suitable for use in preparation of food samples and/or as a rinse during examination of equipment etc. prior to culture. It can also be used as a general purpose diluent in many areas of the laboratory. The addition of glass beads will allow dense material to be broken down and aid the isolation of any bacteria that may be present in “clumps”. This particular product is double wrapped and terminally sterilised by Ethylene oxide. -
Universal Isotonic Saline Solution. -
A medium for the selective enrichment of Salmonellae spp from both clinical and food samples. It is a buffered Lactose Peptone Broth to which Sodium Biselenite is added as the selective agent and Cystine to enhance the recovery of salmonella in low numbers. Subcultures should be made from the top 1/3 of the broth after not more than 24 hours incubation as after this time there is a loss of selectivity. -
A medium for the selective enrichment of Salmonella spp from both clinical and food samples. It is a buffered Lactose Peptone Broth to which Sodium Biselenite is added as the selective agent. Subcultures should be made from the top 1/3 of the broth after not more than 24 hours incubation as after this time there is a loss of selectivity. -
This medium can be used as a screening method for the differentiation of Enterobacteriaceae based on the ability of some species to utilise citrate as a sole source of carbon. It is often used as a screening test for Klebsiella pneumoniae (Positive reaction) while Escherichia coli is negative. Other uses included distinguishing between species of citrate positive Salmonellae (e.g. Salmonella enteritidis) and those that are negative (e.g. Salmonella typhi and Salmonella paratyphi A). The medium is inoculated by stabbing the organism (in pure culture) into the medium. A positive result produces a change of colour from green to bright blue and a negative reaction leaves the colour unchanged. -
This is an aqueous solution of Sodium Hydroxide (4%) suitable for use in the digestion of Sputum samples prior to culture. It is generally used in conjunction with Sputum Neutralising Buffer (BM1325). -
This is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp particularly Mycobacterium bovis. This medium is used primarily in the veterinarian sector. The medium is based on the original formulation of Lowenstein that was subsequently modified by Jensen, the medium contains pyruvate and egg which provide the required protein and fatty acids. It differs from Lowenstein-Jensen Medium in that Sodium Pyruvate has replaced the Glycerol, which has been demonstrated to be inhibitory to some species, particularly M.bovis. The coagulation of the egg albumin during preparation also provides a solid surface for inoculation purposes. Malachite green is incorporated into the medium to inhibit contaminating organisms other than the mycobacteria that may still be present in the specimen after decontamination. Lowenstein-Jensen slopes should be inoculated with pre-treated specimens and incubated at 35-37°C for 8 weeks in 5-10% CO2. Container caps should be left loose for the first week of incubation to allow for circulation of the carbon dioxide as this will help to stimulate growth. Caps should then be tightened to prevent any dehydration of the medium.
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This product may be used as an alternative pre-treatment solution to lessen the background flora of samples before the testing for Mycobacteria species. This reagent should only be used for samples that routinely produce contaminated cultures after processing with an alkaline digestant. -
A nutritious broth medium formulated by Todd and Hewitt for the production of antigenic streptococcal haemolysin. The broth is also used to cultivate streptococci prior to serological grouping. Normally the use of fermentable sugars in the broth would inactivate the haemolysin due to acid production. This is prevented by the use of buffers to maintain the pH in this broth. -
This is a plate count agar originally suggested by the American Public Health Association for the estimation of total viable counts in food and dairy products.
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This is a general-purpose medium supporting a wide range of organisms. It conforms to the requirements of the United States Pharmacopeia for sterility testing of pharmaceutical products as well as being suitable in all areas of bacteriological investigation. -
This is a general-purpose complex medium for the cultivation and isolation of aerobic and anaerobic microorganisms. The base medium, Tryptone Soya Agar (Soybean-Casein Digest agar), conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EP), and Japanese Pharmacopoeia (JP). The medium can be incubated under aerobic or anaerobic conditions after use for sterility testing, air sampling and other areas of bacteriological investigation. -
This is a general-purpose complex medium for cultivation and isolation of fastidious bacteria, yeasts and moulds. The formulation is based on the United States Pharmacopoeia (USP Medium II) and European Pharmacopoeia (EP Medium B). Lecithin and Polysorbate 80 (Tween 80) are added to inactivate surface disinfectants. The medium can be incubated under aerobic or anaerobic conditions for sterility testing, air sampling and other areas of bacteriological investigation. -
Tryptone Soya Broth is also commonly referred to as Soybean-Casein Digest Medium or Tryptic Soya Broth and is abbreviated as TSB. This is a general purpose and nutritious medium for the non-selective enrichment of non-fastidious and some fastidious organisms. Tryptone and Soy peptone are the nitrogen sources in the medium. Glucose is the carbon energy source that facilitates organism growth. Sodium chloride maintains osmotic balance; Di-potassium phosphate is a buffering agent to prevent auto-sterilisation due to acid production during the growth of some organisms. Tryptone Soya Broth formulation conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP). -
A general purpose broth suitable for the cultivation of most micro-organisms including many fastidious organisms and fungi. It is recommended by the United States Pharmacopoeia for sterility testing of many pharmaceutical products. -
This is a general-purpose broth for cultivation of fastidious bacteria, yeasts and moulds. The medium incorporates Polysorbate 80 to act as an emulsifying agent and inactivate phenols. Lecithin is also incorporated to inactivate quaternary ammonium compounds. The medium can be incubated under aerobic or anaerobic conditions for sterility testing, and other areas of bacteriological investigation. -
Tryptone Water is an alternative medium to Peptone Water and more reliable for the testing of Indole production. The medium has a high content of Tryptophan that many organisms, particularly coliforms, break down to form Indole. After incubation add a few drops of Indole reagent to determine the Indole reaction (Red colour is Positive). -
A medium for the enumeration of coliform organisms in food and dairy products. Lactose fermenters produce red/purple colonies often surrounded by a halo of the same colour. Non-lactose fermenters produce pale colonies. Selective agents are Bile salts and crystal violet used to inhibit Gram positive and non-enteric organisms. -
A modification of Violet Red Bile agar designed to give a ‘coliform’ count. In this medium lactose is substituted with glucose. Glucose is fermented by all members of the Enterobacteriaceae thus V.R.B.G.A gives a presumptive Enterobacteriaceae count. Bile salts and crystal violet are used to inhibit Gram positive and non-enteric organisms. The growth of non-fermentative Gram negative bacteria can be suppressed by using the agar overlay method. -
Originally intended for use in surface counting and pour plating techniques in food and dairy products this medium can be used as a general-purpose medium for the cultivation of most non-fastidious organisms.
