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This is a general-purpose medium which supports the growth of a wide range of organisms. It is suitable for Phage Typing, Colicine Typing and for testing the X and V requirements of Haemophilus spp as well as many other areas of bacteriological investigation and conforms to the requirements of the United States Pharmacopeia for sterility testing of pharmaceutical products. -
This is a general-purpose complex medium for the cultivation and isolation of aerobic and anaerobic microorganisms. The base medium, Tryptone Soya Agar (Soybean-Casein Digest agar), conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EP), and Japanese Pharmacopoeia (JP).The medium can be incubated under aerobic or anaerobic conditions for sterility testing, air sampling and other areas of bacteriological investigation. This particular product is triple wrapped and terminally sterilised by Gamma irradiation. Dose Range: 8.0 kGy - 15.0kGy -
Tryptone Soya Broth (Modified) with Novobiocin (20mg/L) This is a selective enrichment broth for the isolation of Escherichia coli 0157, primarily from food and food products, and is capable of detecting the organisms even when they are present in small numbers. It is also increasingly being used in clinical laboratories when screening faecal samples. Based on Tryptone Soya Broth it is made selective for Escherichia coli 0157 by the addition of bile salts and Novobiocin and is also buffered to maintain the pH during incubation. This medium is generally used in conjunction with selective agar subculture (e.g. Sorbitol MacConkey Agar with Cefixime Tellurite – (CT-SMAC)). -
Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the coliform group of organisms. This is one such medium and has been developed primarily as a selective medium for the isolation and enumeration of Escherichia coli in food samples. Based on Tryptone Bile Agar it incorporates the chromogenic agent X-glucuronide, which detects glucuronidase activity, the same enzyme as is detected by MUG reagent. Escherichia coli can be differentiated from other coliform organisms by the presence of glucuronidase resulting in the colonies being blue/green while the other coliforms are colourless. For information on the full technical detail as to the functionality of this medium reference should be made to the many publications available. -
Based on Christensen’s Medium this medium is generally used to detect rapid urease activity of Proteus spp although it can be used to detect urease activity of other Enterobacteriaceae. When used for the later purpose it is necessary to increase the incubation time to as long as 48 hours. -
A modification of Christensen’s Medium by Maslen this medium is generally used to detect rapid Urease activity of Proteus spp although it can be used to detect Urease activity of other Enterobacteriaceae including Urease producing Salmonella and Shigella. Unlike Christensen’s Medium when used for the later purpose it is not necessary to increase the incubation time. -
Violet Red Bile Glucose Agar Violet Red Bile Glucose Agar is a selective medium for the isolation and enumeration of enterobacteriacae in food products. It is a modification of the original Violet Red Bile Agar (PP1150) with the Lactose being replaced with Glucose. As all members of the enterobacteriacae ferment Glucose it allows for a wider range of organisms to be detected. The medium is made selective by the inclusion of Bile Salts and Crystal Violet to inhibit gram-positive and other non-enteric organisms. -
This is a medium for determining the mutagenicity of a chemical reagent using the Ames Test. A histidine requiring strain (His- ) of Salmonella typhimurium is inoculated into a mixture of salt agar, histidine solution and the test reagent, which is mixed and then used to overlay the media. Following incubation if the test reagent is mutagenic it will reverse the His- phenotype allowing growth to occur at a higher level than the control. -
Originally introduced as an aid to recovery of Shigella spp. XLD is also a first class medium for recovery of Salmonella spp. It differs from other media of this type in that it has less Sodium Desoxycholate as its selective agent. The indicator system is somewhat complex taking advantage of the fermentation or otherwise of three carbohydrates (Lactose Sucrose and Xylose) together with Lysine Decarboxylase and Sodium Thiosulphate as an indication of the presence or absence of Hydrogen Sulphide. -
Xylose Lysine Deoxycholate (XLD) Agar is used for the isolation and detection of Salmonella and Shigella spp. It differs from other media of this type in that it has less sodium deoxycholate as its selective agent. The indicator system is somewhat complex taking advantage of the fermentation or otherwise of three carbohydrates (lactose, sucrose and xylose) together with lysine decarboxylase and sodium thiosulphate as an indication of the presence or absence of hydrogen sulphide. The addition of novobiocin (20mg/L) improves the inhibition of Proteus spp. -
A nutrient agar corresponding to the standard formulation described in ISO 6222:1999. -
E&O Laboratories Ltd Yersinia (C.I.N.) Selective Supplement (LS1053) is an antibiotic supplement used to enhance the selective isolation of Yersinia spp. in combination with Yersinia C.I.N. Agar. -
This is a selective medium for the isolation and enumeration of Yersinia spp. in clinical and food samples. It is made selective by the inclusion of Sodium desoxycholate, Crystal violet and the antimicrobials Cefsulodin, Novobiocin and Irgasan. Mannitol is also included which Yersinia ferments giving a colony that produces a ‘Bull’s Eye’ appearance. The majority of other enteric organisms are inhibited but if they do grow they produce a large pinkish colony with an opaque halo. -
b-Nicotinamide-Adenine Dinucleotide (N.A.D.) Supplement E&O Laboratories Ltd N.A.D. Supplement (LS0005) is an enrichment supplement for Haemophilus spp. and Neisseria gonorrhoeae. This supplement can be used in conjunction with sensitivity test agars such as: E&O products PP2148 Iso Sensitivity test agar with 5% Horse Blood and 20mgs/L N.A.D. and PP0972 Mueller Hinton agar with 5 % Horse Blood with 20mgs/L N.A.D.
