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  • This is a selective enrichment broth for the isolation of Salmonella spp. primarily from food and food product samples and conforms to the requirements ISO 6579:2002. It can however be used in other areas including clinical and environmental specimens. Salmonella reduce Tetrathionate and will proliferate in the medium whilst most other enteric organisms are inhibited. Unlike the older traditional Tetrathionate Broth the addition of Novobiocin (40mg/L) improves the inhibition of Proteus spp. Immediately prior to use it is necessary to add 200µl of 2% Iodine/Iodide Solution (BM0946 - Supplied with the medium). Once the Iodine/Iodide Solution has been added the medium should be used immediately and cannot be stored for future use. NB: As this is an opaque medium, the turbidity of the broth alone cannot be used as an indication of growth.
  • This is a selective enrichment broth for the isolation of Salmonellae spp. primarily from food and food product samples and conforms to the requirements as described in ISO 6579:2002. It can however be used in other areas including for clinical and environmental specimens. Salmonella reduce Tetrathionate and will proliferate in the medium whilst most other enteric organisms are inhibited. Unlike the older traditional Tetrathionate Broth the addition of Novobiocin (40mg/L) improves the inhibition of Proteus spp. This complete medium already includes the 2% Iodine solution that is traditionally added immediately before use. NB:  As this is an opaque medium turbidity cannot be used as an indication of growth.
  • A selective medium for the isolation of fungi, particularly dermatophytes from clinical specimens, Mycological Agar is suitable for use in all areas of Mycology. The medium inhibits most bacteria due to the addition of Chloramphenicol which is added to reduce the risk of bacterial contamination when processing material that may be heavily contaminated particularly with Coliforms. Cycloheximide is also added to suppress the growth of yeasts and saprophytic fungi.
  • PP0670

    Nagler Medium

    Based on Fastidious Anaerobe Agar Base with added Egg Yolk Emulsion, this medium can be used to test Clostridium perfringens for phospholipase production. A zone of opalescence around the colonies is indicative of a positive reaction. It can also be used as an aid to identification of Clostridium perfringens if antitoxin is spread onto half of the plate prior to inoculation (Nagler Reaction).
  • Neomycin Selective Supplement E&O Laboratories Ltd Neomycin Selective Supplement (LS0017) is an antibiotic supplement used to enhance the isolation of anaerobes from clinical specimens.  
  • Pages Amoeba Saline & Agar No.1 This is a non-nutrient medium based on Page’s Amoeba Saline, a buffered salt solution, solidified with 1.5% Agar.
  • Novobiocin Supplement (20mgs/L) E&O Laboratories Ltd Novobiocin Selective Supplement can be used to enhance the isolation of Salmonella spp. by motility enrichment and the selective enrichment of E.coli Serogroup O157 in food and faeces samples and also in the isolation of Salmonella typhimurium at 40mgs/L when used with Muller-Kauffmann Tetrathionate (MKTTn) Broth.  
  • PP0690

    Nutrient Agar

    A basic general-purpose medium suitable for use in the cultivation of the less fastidious organisms particularly those that do not require the addition of blood or other enrichment.
  • Oxytetracycline (100mgs/L) Selective Supplement E&O Laboratories Ltd Oxytetracycline Selective Supplement can be used with a glucose, yeast or maltose extract based agar for selective isolation of Yeasts and Moulds from a variety of sample types. Due to the near neutral pH of the medium, Oxytetracycline is added to reduce the risk of bacterial growth when processing materials that may be more heavily contaminated. Media using oxytetracycline as the selective agent is based on the formulation developed by Mossel et al. who stated that the use of this antibiotic in a medium with a neutral pH gave increased counts of yeasts and moulds from a variety of foodstuffs compared with media which relied on a low pH to suppress bacterial growth.
  • This is an established medium, with a neutral pH, used for the enumeration of Yeasts and Moulds
  • PALCAM Selective Supplement E&O Laboratories Ltd PALCAM Selective Supplement (LS0038) is a selective mixture used to supplement PALCAM agar base in order to facilitate the isolation of Listeria monocytogenes and other Listeria species from food samples.  
  • Based on the formulation of Dulbecco Solution ‘A’ this balanced salt solution with added Tween is intended for use primarily in Tissue Culture techniques. It can be used either on its own or with the addition of Calcium and Magnesium salts (Dulbecco Solution B).
  • Plate Count Agar (APHA) (Standard Methods Agar, Tryptone Glucose Yeast Agar) This medium is formulated to A.P.H.A. specification and intended for use in food, dairy and water bacteriology to perform Total Viable Counts. The agar is of high gel strength and is therefore suitable for use in pour plate techniques as well as surface inoculation.
  • This medium is recommended for the detection and enumeration of yeasts and fungi in a variety of sample types. The low pH (5.6) and addition of streptomycin will ensure that the growth of most bacterial species is inhibited and the low mineral content ensures good pigment production by fungi where appropriate.
  • Chromogenic Coliform Agar (CCA) Chromogenic Coliform Agar (CCA) conforms to the ISO 9308-1 guidelines for the detection, enumeration and isolation of coliforms and more specifically Escherichia coli in water samples by the membrane-filtration technique. The colonial differentiation is provided by the chromogenic substrates, Salmon-GAL and X-glucuronide. Salmon-GAL is used for the detection of β-D-galactosidase enzymatic activity. X-glucuronide is used for the detection of β-D-Glucoronidase enzymatic activity. β-D-galactosidase, expressed by all coliforms, cleaves the Salmon-GAL substrate and producing red/pink coloured colonies. Unlike other coliforms, Escherichia coli leaves both Salmon-GAL and X-glucuronide producing a violet/blue coloured colonies. Tryptophan is used to increase detection reliability by improving the indole reaction. The peptones, sodium pyruvate and sorbitol support bacterial growth and simple recovery of sub-lethal thermally injured coliforms. Sodium di-hydrogen phosphate and di-sodium hydrogen phosphate phosphate buffer the medium and sodium chloride is used to achieve osmotic balance. The selectivity is attained by the addition of Tergitol® 7 as it inhibits the growth of Gram positive bacteria.
  • Primary Listeria Selective Supplement E&O Laboratories Ltd Primary Listeria Selective Supplement (LS0210) is a selective mixture used to supplement Primary Listeria Selective Agar (E&O PP7025) in order to facilitate the isolation of Listeria monocytogenes in foodstuffs and other samples.  
  • PP6028

    Primary mLGA

    Traditionally, membrane Lauryl Suphate Broth (mLSB) was used as the standard media for isolating coliforms (including E. coli) from drinking water. Primary membrane Lactose Glucuronide Agar (mLGA) is a chromogenic modification of mLSB formulation aimed at reducing costs by reducing the number of filters used per test sample and aiding in the recovery and identification of coliforms and <em,>E. coli . The medium has been modified from the mLSB formulation by the incorporation of X-glucuronide, sodium pyruvate and agar. X-glucuronide is incorporated to allow for the presumptive isolation of E. coli, sodium pyruvate aids recovery of chlorine stressed organisms and agar is incorporated to remove the need for absorbent pads. This medium is recommended for the enumeration of coliform bacteria and E. coli by a single membrane filtration technique in The Environment Agency’s - The Microbiology of Drinking Water 2009 (Part 4).
  • Tryptone Bile X (TBX) - Glucuronide Agar Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the coliform group of organisms. This is one such medium and has been developed as a selective medium for the isolation and enumeration of Escherichia coli in food samples. Based on Tryptone Bile Agar it incorporates the chromogenic agent X-glucuronide, which detects glucuronidase activity, the same enzyme as is detected by MUG reagent. Escherichia coli can be differentiated from other coliform organisms by the presence of glucuronidase resulting in the colonies being blue/green while the other coliforms are colourless. For information on the full technical detail as to the functionality of this medium reference should be made to the many publications available.
  • Side 1: Primary UTI Chromogenic Agar This is a chromogenic medium based on CLED that has been developed to allow differentiation and presumptive identification of organisms typically found in urinary tract infections. Reduced electrolyte concentration prevents swarming of Proteus spp. A sophisticated binary chromogenic system and supplementation with tryptophan allows differentiation of enterococci (turquoise colonies), Proteus spp (clear colonies with a brown halo), Enterobacter spp (metallic blue colonies), staphylococci (white colonies), and E. coli (purple colonies). Side 2: Columbia Agar w 7% Defibrinated Horse Blood & CNA This is a selective medium for the isolation of Staphylococcus/ spp and Streptococcus spp. It is based on Columbia Agar enriched with defibrinated horse blood which promotes good colony appearance, pigment production and excellent haemolysis from beta-haemolytic streptococci. The medium is made selective by the inclusion of colistin and nalidixic acid to suppress growth of the majority of Gram-negative bacteria.
  • This is a medium for the isolation and identification of Group B streptococci. The principal of the medium is based on the ability of group B streptococci to produce unique orange/red pigmented colonies when incubated anaerobically, particularly on media containing starch products. This medium is non-selective so other organisms will grow on this medium but they do not produce the characteristic pigment.
  • Pseudomonas Agar Base with 1% Glycerol, Cephalothin, Fucidin & Cetrimide (CFC) This is a selective medium for the isolation of Pseudomonas spp primarily in food, water and environmental samples. The medium uses Magnesium and Potassium salts to enhance pigment production and is made selective by the addition of CFC supplement. The presence of blue/green or brown pigmentation or fluorescence is indicative of presumptive Pseudomonas spp. It should be noted however that further testing must be carried out to confirm the full identity of the organism.
  • Pseudomonas Agar Base with 1% Glycerol & CN Supplement A selective medium for the isolation of Pseudomonas aeruginosa the medium is made selective by the inclusion of Cetrimide and Naladixic Acid (CN) supplement to significantly reduce the enteric organisms particularly Proteus and Klebsiella spp. Magnesium and Potassium salts are included to enhance the production of the pigments pyocyanin and fluorescein.
  • Pseudomonas CFC Selective Supplement E&O Laboratories Ltd Pseudomonas CFC Selective Supplement (LS0026) is an antibiotic supplement used to enhance the selective isolation of pseudomonads primarily from food and environmental samples.  
  • E&O Laboratories Limited Pseudomonas Selective CN Supplement (LS0006) is an antibiotic supplement used to enhance the selective isolation of Pseudomonas species particularly Pseudomonas aeruginosa.
  • A selective medium for the isolation of Pseudomonas spp. primarily in food, water and environmental samples the medium is made selective by the addition of CFC supplement (cetrimide, at a concentration of 10mg/L which is said to allow the growth of all pseudomonads, cephalothin and fucidin). The presence of blue/green or brown pigmentation or fluorescence is indicative of presumptive Pseudomonas spp. It should be noted however that further testing must be carried out to confirm the full identity of the organism.
  • PP1281

    R2A Agar

    R2A medium was developed to determine the bacterial count including heterotrophic bacteria in potable waters during treatment and distribution. This medium has a low nutritional content and therefore requires extended incubation times. It is recommended by the Environmental Agency, Methods for the Examination of Waters and Associated Materials, and Standard Methods for the Enumeration of Water and Wastewater.
  • Rappaport Vassiliadis (R.V.) Single Component Enrichment Broth This is an alternative to Selenite and Tetrathionate broths, as a selective enrichment broth for the isolation of Salmonellae spp from food, dairy and environmental samples and is claimed by some workers to be superior to both these formulations. It can also be used in clinical bacteriology but care must be taken to ensure that only a light inoculum is used. Malachite Green and Magnesium Chloride are included in the formulation as selective agents due to their ability to inhibit most enteric organisms but allow salmonellae to multiply freely. NB: This media is not recommended for use when salmonella typhi is suspected.
  • This is a selective enrichment broth for the isolation of Salmonella spp. from pharmaceutical, food, dairy and environmental samples. Malachite Green and Magnesium Chloride are included in the formulation as selective agents due to their ability to inhibit most enteric organisms whilst allowing Salmonella spp. to multiply freely. Gram +ve bacteria and most other enteric bacteria, are typically susceptible to or inhibited by Malachite Green, the high osmotic pressure and/or the low pH of the medium. It should be noted that S.typhi and S.choleraesuis are sensitive to Malachite Green and may therefore be inhibited. This medium conforms to the requirements of the Harmonised USP/EP/JP.
  • RPMI Medium for E-Test RPMI Medium is recommended for use in anti-fungal susceptibility testing of yeasts from clinical isolates using the E-Test method. The medium is based on a simple Glucose Agar with added RPMI-1640 Medium (without Sodium Bicarbonate & Phenol Red), which supplies the necessary vitamins and amino-acids, and MOPS (3-(Morpholino)propanesulfonic Acid) Buffer to maintain the medium pH during incubation.
  • Sabouraud Dextrose Agar with Chloramphenicol (0.5g/L) is a selective media for the isolation of yeasts and fungi suitable for use in all areas of mycology. Sabouraud dextrose agar is a modification of a medium originally described by Sabouraud.(1) The tryptone and meat peptone provides the required nitrogen, carbon and vitamins. The high concentration of dextrose is included as an energy source. Due to the higher pH of the medium, an increased concentration of chloramphenicol is included to improve the selectivity of the media and inhibit a range of Gram-positive and Gram negative bacteria. 1. Sabouraud, R. 1892. Ann. Dermatol. Syphilol. 3:1061.
  • This is one of several media available for the selective isolation of yeasts and fungi suitable for use in all areas of Mycology. The low pH (5.6) of the medium is inhibitory to most bacteria and many diagnostic features such as spores and pigmentation are well developed on this medium. NB: This is a basic medium only and contains no additional supplements.
  • This is a selective medium for the isolation of yeasts and fungi and is particularly suitable for use in sterility testing. The low pH (5.6) of the medium is inhibitory to most bacteria and many diagnostic features such as spores and pigmentation are well developed on this medium. The formulation of this medium conforms to the requirements of the United States Pharmacopoeia & European Pharmacopoeia. This product is wrapped in barrier film to allow for use in Vaporised Hydrogen peroxide sterilisation systems. NB: Final sterilisation of this medium is by Gamma irradiation and it is triple wrapped. Dose Range: Min: 8.0kGy Max: 15.0kGy
  • Sabouraud Dextrose Agar with Chloramphenicol (50mg/L) A selective medium for the isolation of yeasts and fungi, Sabouraud Dextrose Agar is suitable for use in all areas of Mycology. The low pH of the medium inhibits most bacteria, however in this formulation Chloramphenicol (50mg/L) is added to further reduce the risk when processing material that may be heavily contaminated particularly with coliforms.
  • Sabouraud Dextrose Agar with Chloramphenicol (50mg/L) & Cyclohexamide (Actidione) (300mg/L) A selective medium for the isolation of fungi, Sabouraud Dextrose Agar is suitable for use in all areas of Mycology. The low pH of the medium inhibits most bacteria however in this formulation Chloramphenicol (50mg/L) is added to further reduce the risk when processing material that may be heavily contaminated particularly with coliforms. Cycloheximide is also added to suppress the growth of yeasts and saprophytic fungi.
  • This is a modification of Sabouraud Dextrose Agar. The low pH (5.6) of the medium is inhibitory to most bacteria and it has been made specifically selective by the addition of colistin and gentamicin. This further reduces the risk of bacterial contamination when processing material that may be heavily contaminated particularly with Gram negative organisms such as Pseudomonas aeruginosa.
  • This is a selective medium for the isolation of yeasts and fungi and is particularly suitable for use in sterility testing. The low pH (5.6) of the medium is inhibitory to most bacteria and many diagnostic features such as spores and pigmentation are well developed on this medium. The formulation of this medium conforms to the requirements of the United States Pharmacopoeia. Lecithin & Polysorbate 80 (Tween 80) are added to inactivate surface disinfectants (Lecithin neutralises quaternary ammonium compounds & Tween neutralises phenols, formalin, hexachlorophene and in combination with the Lecithin ethanol). NB: Final sterilisation of this medium is by Gamma irradiation and it is triple wrapped. Dose Range: Min: 8.0kGy Max: 15.0kGy
  • Sabouraud Dextrose Agar with Lecithin, Tween 80, Histidine & Sodium Thiosulphate (VHP) (USP) - Irradiated This is a selective medium for the isolation of yeasts and fungi and is particularly suitable for use in sterility testing. The low pH (5.6) of the medium is inhibitory to most bacteria and many diagnostic features such as spores and pigmentation are well developed on this medium. The formulation of this medium conforms to the requirements of the United States Pharmacopoeia. Lecithin, Polysorbate 80 (Tween 80), Histidine and Sodium Thiosulphate are added to inactivate surface disinfectants (Lecithin neutralises quaternary ammonium compounds, Tween 80 and Histidine neutralises phenols, formalin, hexachlorophene and in combination with the Lecithin ethanol and Sodium Thiosulphate inactivate mercurials). This product is wrapped in barrier film to allow for use in Vaporised Hydrogen peroxide sterilisation systems. NB: Final sterilisation of this medium is by Gamma irradiation and it is triple wrapped. Dose Range: Min: 8.0kGy Max: 15.0kGy
  • This product is primarily used as a fixation agent in immunological complement fixation assays. The Sodium citrate acts as an anticoagulant in the Alsever’s solution once it is combined with the sheep blood.
  • Originally intended as a medium for the enumeration of enterococci in water using Membrane Filtration, this medium has become more popular in many other areas such as food bacteriology. The medium contains Tetrazolium Chloride, which is reduced by enterococci to the insoluble red dye Formazan resulting in dark red colonies of enterococci on the agar. It should be noted that this reaction is not exclusive to enterococci and colonies should be confirmed by additional testing e.g. Aesculin hydrolysis.
  • This is a differential medium for the isolation of Escherichia coli 0157:H7. It differs from other MacConkey media in that lactose has been replaced by sorbitol. As Escherichia coli 0157:H7 does not ferment sorbitol, it produces pale translucent colonies whereas most other strains of Escherichia coli do ferment sorbitol and produce pink colonies.
  • Sorbitol MacConkey with Cefixime & Tellurite (CT-Smac) This is a selective differential medium for the isolation of Escherichia coli 0157:H7. It differs from other MacConkey medium in that Lactose has been replaced by Sorbitol. As Escherichia coli 0157:H7 does not ferment Sorbitol it produces pale translucent colonies whereas most other strains of Escherichia coli are Sorbitol positive and produce pink colonies. The medium is made more selective by the addition of the antimicrobial Cefixime and Potassium Tellurite.
  • Staph/Strep Selective Supplement E&O Laboratories Ltd Staph/Strep Selective Supplement (LS0008) is an antibiotic supplement used to enhance the selective isolation of Staphylococci and Streptococci species.  
  • This medium is utilised for the transportation and cryopreservation of Streptococcus pneumoniae & Neisseria meningitidis isolates.
  • Thiosulphate Citrate Bile Salts Sucrose (TCBS) Agar TCBS is a selective isolation medium for culture of pathogenic Vibrio spp. from clinical samples. The formulation was developed by Kobayashi, Enomoto, Skazaki and Kuwahara. This medium inhibits most enterobacteriacae for at least 24 hours. For the isolation of Vibrio spp. other than V.cholerae in environmental bacteriology, it is advisable to incubate at the lower temperature range of 20°C – 30°C. NB - It is not recommended to perform an oxidase test on any presumptive positive isolates directly from TCBS medium.
  • This is a medium to detect Thermo-Stable-Nuclease from Staphylococcus aureus after heat inactivation of the organism. After boiling and centrifugation the supernatant is placed in a well in the plate and incubated for 4 hours. If present, the enzyme breaks down the DNA in the medium and produces a zone of clearing indicating a positive reaction.
  • This medium is based on the formula described by Kupferburg, Johnson and Sprince for the selective isolation of Trichomonas spp. The medium is selective due to the inclusion of the broad spectrum antibiotic, chloramphenicol, to inhibit a wide range of Gram-positive and Gram-negative bacterial species. This medium does not contain an antifungal agent and Candida spp. will not be suppressed. However, the growth of Candida spp. does not interfere with that of Trichomonas spp. The inclusion of methylene blue as a redox indicator allows for the visualisation of any significant oxygen diffusion in the medium. Cultures may be examined microscopically after 48 hours incubation at 37°C for the presence of flagellate protozoans. If a negative result is obtained then the culture may be re-incubated for a further 72 hours.
  • This medium is based on the formula described by Kupferburg, Johnson and Sprince for the selective isolation of Trichomonas spp. The medium is selective due to the inclusion of two selective agents namely, Chloramphenicol and Nystatin, to inhibit a wide range of both Gram-positive and Gram-negative bacterial species as well as yeasts and fungi. The inclusion of methylene blue as a redox indicator allows for the visualisation of any significant oxygen diffusion in the medium. Cultures may be examined microscopically after 48 hours incubation at 37°C for the presence of flagellate protozoans. If a negative result is obtained then the culture may be re-incubated for a further 72 hours.
  • Triphenyltetrazolium Chloride Soya Tryptone (TSAT) Agar Complete Triphenyltetrazolium Chloride (TTC) has been added as an indicator to various media, and recommended by several workers as being helpful in the early recognition and identification of a variety of bacteria including Escherichia coli, Vibrio parahaemolyticus and enterococci. This particular formulation is based on a Tryptone Soya Agar with added Sucrose and is particularly useful when performing counts on food and food product samples. Many of the enterobacteriaceae and enterococci will reduce the TTC to a formazan which colours the colonies deep red making them easier to distinguish and identify. The presence of the Sucrose can also assist in the differentiation of Sucrose fermenting and non-fermenting strains.
  • This is a medium that can be used to differentiate between some of the Enterobacteriacae on the basis of four reactions, fermentation of Lactose, Glucose and Sucrose and the production of H2S. For use the medium is inoculated using a pure culture of the test organism which should be smeared onto the surface of the slope and stabbed into the butt of the medium. For details of the many reactions that may arise during the use of this medium reference should be made to one of the many standard textbooks.
  • This is a plate count agar originally suggested by the American public Health Association for the estimation of total viable counts in food and dairy products.