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This is a medium supporting a wide range of organisms. It conforms to the requirements of the United States Pharmacopeia for antibiotic sensitivity testing of pharmaceutical products. Antibiotic Assay Medium No.32 is a modification of Antibiotic assay medium No.1. (E & O BM4710). This medium is used to develop inoculum of Bacillus subtilis for antibiotic assay used in the test for assaying by the plate assay method. Nutrients and growth factors are supplied by peptic digest of animal tissue, casein hydrolysate, yeast extract, and beef extract. Glucose is a carbon source. -
The principle use for this product is in the testing of disinfectants and antiseptics. In 1989, the European Committee for Standardisation (CEN) set up a technical committee to produce harmonised test methods for disinfectants and antiseptics. The CEN standards provide a useful basis for disinfectant validation, and although alternative methods could be used for assessing disinfectant efficacy, following the same basic methods allows not only direct comparison between products but also comparison across various different laboratories. The adaptability of the methods - numerous validation studies based on the CEN methods have been accepted by both the European and US regulatory authorities - allows end- users to customise the methods to their specific requirements. Lecithin and polysorbate 80 (Tween 80) inactivate surface disinfectants (lecithin neutralises quaternary ammonium compounds and Tween 80 neutralises phenols, formalin, hexachlorophene and in combination with the lecithin ethanol). L-histidine, in combination with lecithin and Tween 80, neutralises aldehydes and formaldehyde generating agents. Sodium thiosulphate neutralises iodine and chlorine. -
Legionellae are more resistant to lower pH and brief exposure to higher temperatures than many other freshwater bacteria. For some water samples with high concentrations of bacteria, it is necessary to use a selective procedure to reduce the number of non-Legionella bacteria before culture. Acid treatment and heating of samples are routinely used for this purpose and to aid antigen extraction. The use of this buffer is recommended in the International Standard ISO 11731-2 "Water quality-Detection and enumeration of Legionella" for this purpose. -
A sterile concentrated emulsion of premium egg yolks, suitable for incorporation in culture media which detect lecithinase production by bacteria. It can be used in media for Bacillus cereus and Staphylococci. Used with serum and Filde’s extract it may be used to produce Nagler plates for Clostridia. -
This is a sterile emulsion of Egg Yolk in Saline containing Potassium Tellurite and is generally used as a selective differential agent in Baird Parker Medium. The complete medium is selective for Staphylococcus aureus as the Potassium Tellurite inhibits most coliform organisms and is also reduced by Staphylococcus aureus to tellurite giving typical black colonies on the Baird Parker Medium.
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A selective medium for the enrichment of Enterobacteriaceae and the detection of Escherichia coli in pharmaceutical products. The inclusion of Bromocresol Purple indicator makes the colour change caused by acid production from the fermentation of lactose easy to read with gas formation. The presence of Ox bile helps to suppress the growth of Gram positive and non-enteric bacterial species. This formulation complies with the Harmonized USP/EP/JP.
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Malt Extract Agar is a medium for the isolation of many yeasts and moulds. The low pH inhibits most bacteria and further selectivity can be achieved by lowering the pH even more by adding lactic acid to the molten cooled, medium. This formulation is customer specific with a request for an additional 5g/L of agar from the customer. It should be noted that excess heating of the medium could result in hydrolysis of the agar resulting in softening of the agar.
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E&O Heat Inactivated Serum is derived from horse serum and is suitable for use in diagnostic assays. One of the reasons for the heat inactivation of serum (heating to 56°C for 30 min) is to inactivate complement, a group of proteins present in serum that are part of the immune response. This is sometimes important for cells that will be used to prepare or assay viruses, used in cytotoxicity assays or other systems where complement may have an unwanted influence. The use of Heat Inactivated Serum is also usually recommended for growing embryonic stem cells. After filtration the dispensing and bottle filling processes are carried out in a state-of-the-art clean room under laminar flow. Once labelled the filled bottles are then subjected to controlled heat inactivation and are frozen and stored at -20°C without delay. The filter sterile Heat Inactivated Horse Serum is supplied in 100 or 500 ml PETG bottles. All E&O products are for in vitro use only. E&O products are intended only for use by qualified professionals who will safely handle and dispose of products they receive. All biologically derived materials (e.g. blood, sera) should be handled as if a potential biohazard. E&O media that contain antibiotics should be handled with care. Chemical resistant gloves, eye protection and laboratory coat should be worn.
