KM0006 – Fastidious Anaerobe Agar

//KM0006 – Fastidious Anaerobe Agar

KM0006 – Fastidious Anaerobe Agar

Fastidious Anaerobe Agar is intended as a primary isolation medium capable of supporting most clinically significant anaerobes. The formulation is complex and includes detoxification agents (starch and sodium bicarbonate), growth enhancing agents (L-Cysteine, arginine, vitamin K, sodium succinate, glucose and pyrophosphate), as well as haemin to encourage pigment production in Bacteroides melaninogenicus. The peptone is the source of the required nitrogen, carbon and vitamins. Glucose also acts as a carbon source and sodium pyruvate as an energy source. Sodium chloride maintains the osmotic balance in the medium. Sodium pyrophosphate acts as a buffering agent and sodium succinate improves the growth of organisms such Bacteroides spp.
Supplementation of the base medium with blood (5 – 10%) will provide additional growth factors for the more fastidious microorganisms, and aids in determining any haemolytic reactions.

Related Supplements :
LS0015 Actinomycete Selective Supplement, LS0017 Neomycin Selective Supplement, LS0022 Clostridium difficile Selective Supplement, LS0023 Clostridium perfringens Selective Supplement, BM0140 Egg Yolk Emulsion

SKU: KM0006 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Clostridium perfringens (with 7% DHB) NCTC 8237 Growth, grey colonies
Bacteroides fragilis (with 7% DHB) NCTC 9343 Growth, grey colonies
Peptostreptococcus anaerobius (with 7% DHB) ATCC 27337 Growth, white/grey colonies

 

Preparation

Suspend 46g/l of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Sterilise at 121°C for 15 minutes. Cool to 45-50°C. Aseptically add the appropriate volume of sterile defibrinated horse or sheep blood. Various selective supplements, such as neomycin (LS0017), may also be aseptically added at this point to create a variety of selective blood agar products. Gently homogenize the final medium and dispense into appropriate sterile containers.

Formulation

FORMULA* g/L
Peptone 23.0
Soluble starch 1.0
Sodium chloride 5.0
Sodium bicarbonate 0.4
Glucose 1.0
Sodium pyruvate 1.0
L-Cysteine HCl 0.5
L-Arginine 1.0
Haemin 0.01
Vitamin K 0.001
Soluble pyrophosphate 0.25
Sodium succinate 0.5
Agar 12.0
Total 46 46
* Adjusted / supplemented as required to meet performance requirements