CLED Double Indicator Agar (Bevis)

///CLED Double Indicator Agar (Bevis)

CLED Double Indicator Agar (Bevis)

KM0005 CLED Double Indicator Agar (Bevis) is a differential culture medium used clinically for isolation and enumeration of bacteria in urine, from the suspected cases of urinary tract infection (UTI).

KM0005 CLED Double Indicator Agar (Bevis) is a differential culture medium which contains cystine, lactose, bromothymol blue, acid fuchsin and is electrolyte deficient to prevent swarming of Proteus species. Bevis changed the original medium by Mackey and Sandys by introducing a second pH indicator to enhance the differentiation of colony characteristics of lactose and non-lactose fermenting organisms. The medium can allow for quantitative determination of urinary pathogens, including Proteus species, when automated systems or calibrated loops are used for inoculation.

KM0005 is recommended as a differential primary isolation media by the UK Standards for Microbiology Investigations and tested in accordance with ISO 11133:2014.

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated pH

Prepared pH

Product Description

Organisms Ref. No Result
Staphylococcus aureus NCTC 12981 Growth: Yellow/orange colonies with red halo
Escherichia coli NCTC 12241 Growth: Orange colonies with red halo
Proteus mirabilis NCTC 10975 Growth: Blue colonies (no swarming)
Pseudomonas aeruginosa NCTC 12903 Growth: Blue colonies
Klebsiella pneumoniae NCTC 9633 Growth: Yellow colonies
Enterococcus faecalis NCTC 12697 Growth: Pale yellow colonies
Proteus vulgaris ATCC 8427 Growth: Blue colonies (no swarming)

 

Formula*

Formula * g/L
Peptone 8.0
Beef extract 3.0
Lactose 10.0
L-cystine 0.0128
Anrade’s Indicator 0.08
Bromothymol Blue 0.02
Agar 15.0
Total 36.2
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 36.2g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes Heat with frequent agitation and boil for one minute to completely dissolve the medium. Sterilise at 121°C for 15 minutes. Cool to 45-50°C and aseptically dispense into appropriate sterile containers.