Fine Powder – Page 6 – E & O Laboratories Ltd

KM0215
Half Fraser Broth Complete
KM0215 Half Fraser Broth Complete is used to make Half Fraser Broth enrichment media for the isolation of Listeria species from food and environmental samples.

Developed in 1988 by Fraser ⁽¹⁾, the formulation can be made as primary enrichment Half Fraser Broth and secondary enrichment Fraser Broth according to the requirements of ISO 11290-1:2017 ⁽²⁾. KM0215 is recommended by the International Standardization Organization ⁽²⁾ and tested in accordance with the principals of ISO 11133:2014 ⁽³⁾.

The nalidixic acid, acriflavine, and ferric ammonium citrate are already included in the formulation, so no additional supplements are required to make complete Half Fraser Broth.
1. Fraser, J. and Sperber, W. (1988) Rapid Detection of Listeria spp. in Food and Environmental Samples by Esculin Hydrolysis. Journal of Food Protection, 51(10), pp.762-765. 2. International Standardization Organization (2017) 11290-1:2017 Microbiology of the food chain - Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. - Part 1: Detection method. Geneva: ISO. 3. International Standardization Organization (2014) 11133:2014 Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media. Geneva: ISO.
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KM0032
Hektoen Enteric Agar

Hektoen enteric agar was developed by King and Metzger as a differential selective medium for the isolation of Shigella spp. and Salmonella spp. species from enteric pathological samples. The meat peptone and yeast extract provide the required nitrogen, carbon and vitamins. Lactose, sucrose and salicin are fermentable carbohydrates. Bromothymol blue is added as a pH indicator in order to identify carbohydrate fermenting organisms. The combination of ferric ammonium citrate and sodium thiosulfate allows the production of hydrogen sulphide. Hydrogen sulphide positive colonies produce black centred colonies. Sodium chloride maintains the osmotic balance. The bile salts and acid fuchsin inhibit Gram-positive organisms.

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KM0009
Helicobacter Pylori Agar

This is a selective medium for the isolation of Helicobacter pylori from clinical samples. The medium is based on a modification of Campylobacter CCDA Blood Free Medium with charcoal, ferrous sulphate and sodium pyruvate replacing the horse blood. The medium is made selective by the addition of vancomycin and cefsulodin, to suppress other bacteria, and amphoteracin to inhibit yeasts. Horse serum (10%) is also added to promote optimum growth of Helicobacter spp. Related Supplements : LS0031 Helicobacter pylori Selective Supplement, SHS500 Sterile Horse Serum 500ml

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KM0073
Hoyle’s Agar

Hoyles agar is selective culture medium for the isolation and differentiation of Corynebacterium diphtheriae types. Hoyles agar allows for rapid growth of the organisms and normally 18 hours incubation should be sufficient for a diagnosis. As the medium is highly selective, inoculation should be by rubbing the swab (or other material) over the entire surface of the agar, there is no need to spread the inoculum with a loop indeed doing so can cause the organism to be missed especially when they are present only in small numbers. The Elek method can be used to determine the toxigenicity of any C. diptheriae strains. The beef extract and peptone act as a source of nitrogen, carbon and vitamins. The sodium chloride maintains osmotic balance. This medium should be used in conjunction with two supplements: lysed horse blood at 50 ml/l and 10ml/l of potassium tellurite 3.5% solution (BM2230). Lysed horse blood provides added nutrients to the medium and potassium tellurite inhibits Gram-negative and several Gram-positive microorganisms. Related Supplements : BM2290 3.5% Potassium Tellurite Solution, Horse Blood Lysed

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KM0111
Kligler Iron Agar

Kligler iron agar is used to differentiate between some of the enterobacteriacae on the basis of three reactions: fermentation of lactose and glucose and the production of hydrogen sulphide. Kligler iron agar is a modification of the original formulation developed by Kligler. It incorporates the principles of Russell’s double sugar agar with Kligler ‘s lead acetate agar. The peptone provides the required nitrogen, carbon and vitamins. Lactose and glucose are carbohydrates. Acid production from their fermentation is detected by the phenol red pH indictor. Sodium thiosulphate is reduced to hydrogen sulphide which is detected by the ferric citrate indicator. Sodium chloride maintains the osmotic balance.

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KM8008
Lactalbumin Hydrolysate

Lactalbumin hydrolysate is obtained through enzymatic hydrolysis of lactalbumin protein in milk whey. This product is rich in essential amino acids as well as providing a source of nitrogen, carbon and vitamins. It is commonly utilized in media for tissue culture and for the production of vaccines as well as being useful for bacterial and fermentation media.

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KM0116
Lactose Broth

Lactose broth is used for the performance and confirmation of the Presumptive Test for coliforms in water and dairy samples. This medium is also frequently used as a pre-enrichment medium when testing foods, water samples and dairy products for Salmonella spp. Beef extract and gelatin peptone provide the required carbon, nitrogen and vitamins in this medium. Lactose is a fermentable carbohydrate. Fermentation of lactose is detected by the production of gas

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KM0117
Lauryl Tryptose Agar

Lauryl Tryptose Broth is a selective medium for the detection of coliforms in water and wastewater. This formulation is based on the Mallmann and Darby formulation. (1) The tryptone provides the required carbon, nitrogen and vitamins in this medium. Lactose is a fermentable carbohydrate. The sodium chloride maintains the osmotic balance whilst the dipotassium hydrogen phosphate and potassium hydrogen phosphate act as buffers. Sodium lauryl sulphate is a selective again used to inhibit non-coliforms. References (1) Mallmann, W. L. and Darby, C. W. 1941. Uses of a lauryl sulphate tryptose broth for the detection of coliform organisms. Am J. Public Health. 31:127

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