Lactose Broth

Lactose Broth

SKU: KM0116 Category:

Lactose broth is used for the performance and confirmation of the Presumptive Test for coliforms in water and dairy samples. This medium is also frequently used as a pre-enrichment medium when testing foods, water samples and dairy products for Salmonella spp.
Beef extract and gelatin peptone provide the required carbon, nitrogen and vitamins in this medium. Lactose is a fermentable carbohydrate. Fermentation of lactose is detected by the production of gas

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Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

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Dehydrated PH

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Product Description

Organisms Ref. No Result
Escherichia coli NCTC 12241 Turbid growth, gas production



Beef extract 3.0
Gelatin peptone 5.0
Lactose 5.0
Total 13.0
*Adjusted/supplemented as required to meet performance requirements


Suspend 13g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes with frequent stirring to completely dissolve the medium. Cool to 45-50°C and dispense into appropriate sterile containers.