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Prepared from Minced Meat granules overlaid with Fastidious Anaerobe Broth this medium is suitable for the recovery, isolation and storage of the most fastidious anaerobes and is possibly one of the best variations of Cooked Meat Medium available. Fastidious Anaerobe Broth is designed for optimum growth of all anaerobes, with the growth factors Vitamin K, Haemin and L-Cysteine included in the medium to assist those anaerobes that require them. L-Cysteine together with Sodium Thioglycollate is also present to reduce the ph of the medium and a small amount of Agar to reduce the absorption of Oxygen and convection currents. The redox indicator Resazurin is also present. If immediately before use a narrow band of reddish/purple is apparent at the surface of the broth this does not indicate that the medium is unsuitable for use. This is due to the action of oxygen on the redox indicator and the medium should be placed in a boiling water bath, with the cap loosened, for about 15 minutes to remove dissolved oxygen. Immediately on removal from the water bath the cap should be tightened and the medium allowed to cool without agitation. -
Prepared from Minced Meat granules overlaid with Brain Heart Infusion Broth this medium is suitable for recovery, isolation and storage of the most fastidious anaerobes as well as many aerobes. -
This is a defined medium primarily for the cultivation of fungi and bacteria that are capable of utilising Sodium Nitrate as their sole source of Nitrogen. If made more acidic by adjusting to pH 3.5 it can also be used for isolation of yeasts. It has also been used for the identification of Candida albicans by chlamydospore production. -
All E&O products are for in vitro use only. E&O products are intended only for use by qualified professionals who will safely handle and dispose of products they receive. All biologically derived materials (e.g. blood, sera) should be handled as if a potential biohazard. E&O media that contain antibiotics should be handled with care. Chemical resistant gloves, eye protection and laboratory coat should be worn. -
Legionellae are more resistant to lower pH and brief exposure to higher temperatures than many other freshwater bacteria. For some water samples with high concentrations of bacteria, it is necessary to use a selective procedure to reduce the number of non-Legionella bacteria before culture. Acid treatment and heating of samples are routinely used for this purpose and to aid antigen extraction. The use of this buffer is recommended in the International Standard ISO 11731-2 "Water quality-Detection and enumeration of Legionella" for this purpose. -
For in vitro diagnostic use. BM1682 Faecal Transport Solution is recommended for the transport of clinical specimens, especially those associated with enteric pathogens (1). This formulation allows for transport at chilled or ambient temperatures.
BM1682 Faecal Transport Solution is a modified Cary Blair Transport Medium (2), designed for the transportation and preservation of clinical specimens, primarily stool and rectal swabs. The product is designed to maintain the viability of enteric bacterial pathogens during transport and subsequent storage at chilled or ambient temperatures.
Faecal Transport Solution is an isotonic, buffered, and nonnutritive medium with a carefully balanced composition. It includes agar to help reduce oxygen diffusion, sodium thioglycolate to impede oxidation, and disodium phosphate to help maintain pH. The relatively high pH of the medium helps to minimises overgrowth of non-target organisms and prevent acid formation in the specimen. References1. CLSI M40-A2. (2014). Quality control of microbiological transport systems; Approved standard – _second edition. CLSI document M40-A2. Wayne, PA: Clinical and Laboratory Standards Institute. 2. Cary, S.G. and Blair, E.B. (1964). New Transport Medium for Shipment of Clinical Specimens. J. Bact, 88:96-98.
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A medium designed for optimum growth of anaerobes, particularly the fastidious organisms, it is generally used as an enrichment broth and can be used as a Blood Culture Medium. The growth factors Vitamin K, Haemin and L-Cysteine are included in the medium to assist those anaerobes that require them. L-Cysteine together with Sodium Thioglycollate reduces the ph of the medium and the Agar is present to reduce the absorption of Oxygen and convection currents. The redox indicator Resazurin is also present. -
A medium designed for optimum growth of anaerobes, particularly the fastidious organisms, it is generally used as an enrichment broth and can be used as a Blood Culture Medium. The growth factors Vitamin K, Haemin and L-Cysteine are included in the medium to assist those anaerobes that require them. L-Cysteine together with Sodium Thioglycollate reduces the ph of the medium and the Agar is present to reduce the absorption of Oxygen and convection currents. The redox indicator Resazurin is also present. The addition of glass beads will allow for the maceration of the sample and aid the homogenisation of the suspension. -
A medium for the selective isolation of group B streptococci. This information has been obtained directly from the HPA files at the customers request. -
This is a chemical complex which should be used in conjunction with BM0945 Muller – Kauffmann Tetrathionate Broth Base. It is recommended that the complex is used as a 2% solution with the base media and should be only added on the day of use. -
Kirchner medium is a liquid medium for the selective enrichment and isolation of Mycobacteria spp from clinical specimens, particularly when the organisms may be present only in small numbers (e.g. CSF and tissue biopsies). This medium has been enriched by the addition of calf serum as it is a widely used supplement because it is rich in growth factors. The medium is made selective by the inclusion of Polymyxin B, Ticarcillin and Trimethoprim to inhibit other bacteria and Amphotericin B to inhibit yeasts and fungi. It is generally used in conjunction with a solid medium such as Lowenstein Jensen Medium. Specimens from normally sterile body sites may be inoculated without digestion and decontamination. Other specimens should be pre-treated according to standard procedures. The inoculated medium should be incubated at 35-37°C for up to 8 weeks. Kirchner medium should be used in conjunction with a solid medium (e.g. Lowenstein-Jensen medium) in order to accelerate differentiation and identification testing.
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Product description to follow.
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Luria Bertani broth (LB Broth) is a nutrient broth primarily used for the growth and maintenance of Escherichia coli. Used as the primary propagation step for donor or recipient cells, when further work is to be performed on LB Agar. -
Letheen Broth with Neutraliser and 1% Tween 80 is primarily intended for use in assessing the bactericidal activity of quaternary ammonium compounds and determining the phenol coefficient of cationic surfactants. It can also be used in environmental testing, particularly in areas subjected to surface disinfection. Lecithin and polysorbate 80 (Tween 80) inactivate surface disinfectants (lecithin neutralises quaternary ammonium compounds and Tween 80 neutralises phenols, formalin, hexachlorophene and in combination with the lecithin ethanol).
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LIM Broth with 10% Serum A nutritious, selective broth medium utilising the base formulation developed by Todd and Hewitt for the enrichment of Group B Streptococci. The LIM broth is made selective by the inclusion of Colistin and Nalidixic Acid to suppress the growth of the majority of other bacteria. Serum is also included to enhance the nutritional qualities of the base medium. -
This is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp., particularly Mycobacterium tuberculosis, from clinical samples. Based on the original formulation of Lowenstein that was subsequently modified by Jensen, the medium contains glycerol and egg which provide the required protein and fatty acids. The coagulation of the egg albumin during preparation also provides a solid surface for inoculation purposes. Malachite green is incorporated into the medium to inhibit contaminating organisms other than the mycobacteria that may still be present in the specimen after decontamination. Lowenstein Jensen Medium slopes should be inoculated with pre-treated specimens and incubated at 35-37°C for 8 weeks in 5-10% CO2. Container caps should be left loose for the first week of incubation to allow for circulation of the carbon dioxide as this will help to stimulate growth. Caps should then be tightened to prevent any dehydration of the medium
