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This is a selective medium for the isolation of ESBL (Extended Spectrum Beta-Lactamase) producing strains of Escherichia coli. It should be noted that AmpC isolates may also be detected on this medium whilst non - ESBL organisms will be inhibited on this medium. The inclusion of bromocresol purple indicator displays the acid production due to the lactose fermentation by means of a colour change from purple to yellow. N.B. – This is double strength broth. -
Selenite Mannitol Broth is an alternative to Selenite F Broth for the selective enrichment of Salmonellae spp from Clinical, Food and Environmental specimens. Based on Buffered Mannitol Broth, it is made selective by the addition of Sodium Biselenite. The fermentation of Mannitol by Salmonellae is said to correct the alkaline pH swing which can occur during incubation. Following overnight incubation subculture(s) are usually made on to one or more of the many selective enteric solid media. -
This is an agar-based medium for the isolation of Mycobacterium spp. from veterinary samples; particularly the species primarily responsible for bovine TB, M.bovis. The medium is complex but includes L-Glutamic acid, Ammonium sulphate, Sodium citrate, Pyridoxine and Biotin as growth factors and Magnesium sulphate, Ferric ammonium citrate as sources of trace ions. Di-sodium phosphate and Mono-potassium phosphate are also present to maintain the pH of the medium. Further enrichment is provided by the addition of Oleic acid, Albumin and Dextrose. The medium is made selective by the inclusion of Ticarcillin, Polymyxin B, Trimethoprim and Amphotericin B. Malachite green is also incorporated to provide a colour contrast between the colonies and the medium as well as contributing some inhibitory effect on organisms other than mycobacteria. It should be noted that Glycerol is NOT added to this medium as Glycerol can be inhibitory for M.bovis when examining veterinary samples. However, this product contains Lysed sheep blood, Adult bovine serum and Sodium pyruvate to enhance the growth of M.bovis. -
This medium is recommended by BSI and ISO for the enumeration of viable organisms in milk and other dairy products. It can also be used as a general-purpose medium for the cultivation of most organisms, particularly those that are less fastidious in their nutritional requirements.
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Modified Semi Solid Rappaport Vassiliadis Medium (MSRV) is a modification of Rappaport-Vassiliadis enrichment broth for detecting motile Salmonella spp. in faeces and food products. The original research on MSRV Medium revealed a semi-solid could be used as a rapid and sensitive test for isolating motile Salmonella spp. from food products following pre-enrichment or selective enrichment. The semi-solid medium allows motility to be detected as halos of growth around the original point of inoculation.
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MSS is a viral transport solution designed to facilitate collection and transport of viruses safely from site of sampling to a laboratory location at ambient temperatures. The medium is buffered to help maintain a pH of 7.1, and contains a metal ion chelator, a non-ionic surfactant and a chaotropic agent to ensure disruption of virus particles, denaturation of viral proteins and inactivation of nucleases that may destroy target nucleic acids. The resultant sample is suitable for virus identification by PCR analysis as well as other nucleic acid manipulations. MSS is designed to disrupt viral particles, denature viral proteins and, therefore, reduce the infection hazard during transport and laboratory processing. NB – BM1677 MSS has been validated only for use with the SARS-CoV-2 virus 2ml in self-standing M043 Tube • Aseptically filled • Red cap with swab capture point • Storage: 2-30°C • Store in the dark • Shelf life: 730 days • Samples collected in MSS are suitable for PCR analysis and other nucleic acid manipulations. • MSS is designed to disrupt viral particles, denature viral proteins, inactivate nucleases and reduce the infection hazard during transport and laboratory processing. -
This is best described as a multi-purpose medium for differentiation of enterobacteriacae that combines three individual tests into a single medium. For use the medium is inoculated by making a single stab into the medium with a straight wire (or equivalent) using a pure culture (or discrete single colony) of the test organism. Following incubation it is recommended that the medium should first of all be examined to determine whether or not the organism is motile. The presence of motility is apparent by the organism tracking out from the line of inoculation and often turning the medium turbid. Non-motile organisms generally grow within the stab line leaving the surrounding medium clear. Urease positive organisms (e.g. Proteus spp) turn the medium bright red due to the hydrolysis of the Urea in the presence of the Phenol Red Indicator often making it difficult to determine the other parameters.Indole is tested for by layering a small amount of Indole Reagent (Erlich’s or Kovac’s appear to work equally well) onto the surface of the medium and allowed a few minutes to react. A positive result is indicated by the formation of a red line at the interface of the reagent and the medium. -
This is a solution of novobiocin used as the selective agent in Modified Semi Solid Rappaport Vassiliadis Medium (BM4031) for the detection of motile Salmonella spp.
