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Maximum Recovery Diluent (Peptone/ Saline Diluent) An osmotically controlled solution for the preparations of suspensions of food samples and for use as a diluent in dilution techniques for bacterial enumeration. The presence of a low level peptone lessens the physiological shock normally experienced by bacterial cells when they are introduced to a diluent such as Ringers Solution. The level of peptone is such that multiplication of the organisms is not possible in the time in which the sample will be present in the diluent. -
For the enumeration of Escherichia coli and coliform organisms in water using a Membrane Filtration Technique. Previously known as Membrane Enriched Teepol Broth, Lauryl Sulphate has replaced Teepol 610, which is no longer available. Phenol Red is included in the medium making it possible for coliforms to be more readily detected following incubation. -
This is a liquid medium for growing pure cultures of Mycobacterium spp., including M. tuberculosis, for use in antimicrobial assays and biochemical tests. The medium is complex but includes L-Glutamic acid, ammonium sulphate, sodium citrate, pyridoxine and biotin as growth factors as well as magnesium sulphate and ferric ammonium citrate as sources of trace ions. Di-sodium phosphate and Mono-potassium phosphate are also present to maintain the pH of the medium. The medium is further enriched by the addition of the Middlebrook OADC Enrichment supplement. OADC contains oleic acid to provide fatty acids for growth promotion, bovine albumin and catalase as protective compounds as well as sodium chloride and dextrose.
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This medium is recommended by BSI and ISO for the enumeration of viable organisms in milk and other dairy products. It can also be used as a general-purpose medium for the cultivation of most organisms, particularly those that are less fastidious in their nutritional requirements.
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A broth suitable for the cultivation of bacteria especially members of the Enterobacteriacae genus. This medium allows for the detection of gas when a Durhum tube is incorporated in the medium. -
A broth suitable for the cultivation of bacteria especially members of the Enterobacteriacae genus. Which allows the detection of acid & gas when a Durhum tube is incorporated in the medium. -
Modified Semi Solid Rappaport Vassiliadis Medium (MSRV) is a modification of Rappaport-Vassiliadis enrichment broth for detecting motile Salmonella spp. in faeces and food products. The original research on MSRV Medium revealed a semi-solid could be used as a rapid and sensitive test for isolating motile Salmonella spp. from food products following pre-enrichment or selective enrichment. The semi-solid medium allows motility to be detected as halos of growth around the original point of inoculation.
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This is best described as a multi-purpose medium for differentiation of enterobacteriacae that combines three individual tests into a single medium. For use the medium is inoculated by making a single stab into the medium with a straight wire (or equivalent) using a pure culture (or discrete single colony) of the test organism. Following incubation it is recommended that the medium should first of all be examined to determine whether or not the organism is motile. The presence of motility is apparent by the organism tracking out from the line of inoculation and often turning the medium turbid. Non-motile organisms generally grow within the stab line leaving the surrounding medium clear. Urease positive organisms (e.g. Proteus spp) turn the medium bright red due to the hydrolysis of the Urea in the presence of the Phenol Red Indicator often making it difficult to determine the other parameters.Indole is tested for by layering a small amount of Indole Reagent (Erlich’s or Kovac’s appear to work equally well) onto the surface of the medium and allowed a few minutes to react. A positive result is indicated by the formation of a red line at the interface of the reagent and the medium. -
de Man, Rogosa & Sharpe (MRS) Agar M.R.S. Agar is intended for the cultivation and enumeration of Lactobacillus spp from a variety of sources and can be used as an alternative to Orange Serum Agar for that purpose. Magnesium Sulphate, Manganese Sulphate, Sodium Acetate and Tween are included as growth supplements. The medium can be made more specific for lactobacilli generally by lowering the pH to between 5.0 and 5.5. This has the effect of inhibiting most streptococci that may otherwise grow on the medium and can be readily confused with lactobacilli. -
This is a solution of novobiocin used as the selective agent in Modified Semi Solid Rappaport Vassiliadis Medium (BM4031) for the detection of motile Salmonella spp.
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This is a selective enrichment broth for the isolation of Salmonella spp. primarily from food and food product samples and conforms to the requirements ISO 6579:2002. It can however be used in other areas including clinical and environmental specimens. Salmonella reduce Tetrathionate and will proliferate in the medium whilst most other enteric organisms are inhibited. Unlike the older traditional Tetrathionate Broth the addition of Novobiocin (40mg/L) improves the inhibition of Proteus spp. Immediately prior to use it is necessary to add 200µl of 2% Iodine/Iodide Solution (BM0946 - Supplied with the medium). Once the Iodine/Iodide Solution has been added the medium should be used immediately and cannot be stored for future use. NB: As this is an opaque medium, the turbidity of the broth alone cannot be used as an indication of growth. -
This is a selective enrichment broth for the isolation of Salmonellae spp. primarily from food and food product samples and conforms to the requirements as described in ISO 6579:2002. It can however be used in other areas including for clinical and environmental specimens. Salmonella reduce Tetrathionate and will proliferate in the medium whilst most other enteric organisms are inhibited. Unlike the older traditional Tetrathionate Broth the addition of Novobiocin (40mg/L) improves the inhibition of Proteus spp. This complete medium already includes the 2% Iodine solution that is traditionally added immediately before use. NB: As this is an opaque medium turbidity cannot be used as an indication of growth. -
This is a general-purpose neutralising diluent used particularly in the pharmaceutical industry. Lecithin, L-histidine and Tween 80 are present to inactivate surface disinfectants such as quaternary ammonium compounds, phenols, aldehydes (including formaldehyde), hexachlorophene and ethanol. The diluent may be used in sampling surfaces and equipment (including endoscopes) to detect the presence of surviving microorganisms after disinfection. The presence of the surfactant Tween 80 also helps release adherent organisms from surfaces being tested.
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This is a non-nutrient medium based on Page’s Amoeba Saline, a buffered salt solution, solidified with 1.5% Agar.
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A general purpose medium for the cultivation of organisms that are less fastidious in their nutritional requirements. This particular formulation has additional 0.2% Agar added to provide for a firmer medium without loss of efficacy. Generally used to maintain cultures or to check the purity of subcultures from isolation media. -
A general-purpose medium for the cultivation of organisms that are less fastidious in their nutritional requirements. -
Based on Nutrient Broth with an additional 6.5% Sodium Chloride (Total Sodium Chloride content = 7%) this medium is suitable for use in the investigation of outbreaks involving Staphylococcus aureus including MRSA. The additional Sodium Chloride inhibits most other organisms allowing the staphylococci to multiply freely even if present in small numbers. The medium is generally used as an enrichment medium in conjunction with subculture onto selective solid media. -
A general-purpose medium that can be used as a base for carbohydrate fermentation media. It has a high level of Tryptone and is therefore also suitable for use in Indole testing. -
This is one of the large group of media, affectionately known as ‘Peptone Water Sugars’, that are generally used in the screening and/or identification of organisms particularly the enterobacteriacae. A positive fermentation of the substrate is clearly indicated by the medium turning pink due to the inclusion Andrade’s Indicator. -
This is a base medium to which can be added selective supplements of choice for the presumptive identification and enumeration of Clostridium perfringens in food products using poured plate techniques. Sodium metabisulphite and Ferric ammonium citrate are included in the base and together provide an indicator of sulphite reaction by Clostridium perfringens, which produces black colonies on the medium. It is recommended that this medium be used with an overlay otherwise cultures may not grow as black colonies. NB: This is a base medium only and contains no selective supplements. -
Based on the formulation of Dulbecco Solution ‘A’ this balanced salt solution is intended for use primarily in Tissue Culture techniques. It can be used either on its own or with the addition of Calcium and Magnesium salts (Dulbecco Solution B). -
Originally intended for use in surface counting and pour plating techniques this medium can be used as a general purpose medium for the cultivation of most organisms particularly those that are less fastidious in their nutritional requirements. Can also be used as a maintenance medium for stock cultures. -
This medium is recommended for the detection and enumeration of yeasts and moulds in food and dairy products. It can also be used for the cultivation of fungi although with the prolonged incubation necessary cultures may become overgrown by bacteria. The low pH (5.6) suppresses the growth of most bacteria and the low mineral content ensures good pigment production by fungi where appropriate. -
Traditionally, standard methods for enumeration of heterotrophic bacteria in water have used nutritionally rich media with incubation at 35°C. This may represent only a small percentage of bacteria present in the sample. R2A agar is a nutritionally reduced medium which, when combined with incubation at lower temperatures for longer time periods, results in enhanced recovery of stressed and chlorine damaged organisms from treated waters resulting in higher more realistic bacterial counts.
