Pharmaceutical/Industrial & Cosmetic Bottled & Bagged Media List
Bottled & Bagged ready to use culture media for the food, water and environmental microbiological testing laboratories: Broths, agars and diluents are dispensed into various containers and volumes using both manual and fully automated microbiology laboratory equipment production lines. An extensive range of containers/volumes and microbiological media formulations are on offer to meet your individual requirements.
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This is a medium supporting a wide range of organisms. It conforms to the requirements of the United States Pharmacopeia (USP) for antibiotic sensitivity testing of pharmaceutical products. As previously stated Antibiotic Medium No. 1 is used in the performance of antibiotic assays. This medium is prepared according to the specifications detailed in the USP. The use of this medium assures well-defined inhibition zones of the test organisms. Nutrients and growth factors are supplied by peptic digest of animal tissue, casein hydrolysate, yeast extract, and beef extract. Glucose is a carbon source. -
This is a medium supporting a wide range of organisms. It conforms to the requirements of the United States Pharmacopeia for antibiotic sensitivity testing of pharmaceutical products. Antibiotic Assay Medium No.32 is a modification of Antibiotic assay medium No.1. (E & O BM4710). This medium is used to develop inoculum of Bacillus subtilis for antibiotic assay used in the test for assaying by the plate assay method. Nutrients and growth factors are supplied by peptic digest of animal tissue, casein hydrolysate, yeast extract, and beef extract. Glucose is a carbon source. -
This is a medium supporting a wide range of organisms. It conforms to the requirements of the United States Pharmacopeia for antibiotic sensitivity testing of pharmaceutical products. Antibiotic Assay Medium No. 8 is recommended for preparing inoculum of Bacillus subtilis to be used as a test for assaying Vancomycin by plate assay method. -
BM0070 Brain Heart Infusion Broth is a nutritious, isotonic, general-purpose medium suitable for the isolation of most micro-organisms including many fastidious organisms and, with enrichment as appropriate, is suitable as a base for blood culture medium. Modern BHI typically uses an infusion from porcine brains and hearts rather than calf brain tissue, and uses disodium phosphate as a buffer, rather than the calcium carbonate used by Rosenow and Haden. It is a nutrient-rich medium and can therefore be used to culture a variety of fastidious organisms, including streptococci, pneumococci, and meningococci, which can be challenging to grow. Brain Heart Infusion Broth is recommended by the FDA BAM for the enrichment for pathogenic Escherichia coli in foods and environmental samples, and as a medium used in the coagulase test for the identification of Staphylococcus aureus from foods, and Gram-positive cocci from cosmetic samples. NB: Organisms that produce large amounts of acid in the medium may overwhelm the buffering system and as a result ‘auto-sterilise’ the culture. -
Buffered Sodium Chloride Peptone Diluent is used for the microbial examination of pharmaceutical products, e.g. as a diluent for sample preparation or as a rinsing solution. This formulation complies with the Harmonized USP/EP/JP. -
The principle use for this product is in the testing of disinfectants and antiseptics. In 1989, the European Committee for Standardisation (CEN) set up a technical committee to produce harmonised test methods for disinfectants and antiseptics. The CEN standards provide a useful basis for disinfectant validation, and although alternative methods could be used for assessing disinfectant efficacy, following the same basic methods allows not only direct comparison between products but also comparison across various different laboratories. The adaptability of the methods - numerous validation studies based on the CEN methods have been accepted by both the European and US regulatory authorities - allows end- users to customise the methods to their specific requirements. Lecithin and polysorbate 80 (Tween 80) inactivate surface disinfectants (lecithin neutralises quaternary ammonium compounds and Tween 80 neutralises phenols, formalin, hexachlorophene and in combination with the lecithin ethanol). L-histidine, in combination with lecithin and Tween 80, neutralises aldehydes and formaldehyde generating agents. Sodium thiosulphate neutralises iodine and chlorine. -
Dey & Engley Broth has the ability to neutralise antimicrobial chemicals and is used for environmental sampling for the detection and enumeration of microorganisms present in cosmetic and/or environmental samples. In this formulation the broth has been modified by the addition of Tween 80 (Polysorbate 80), Lecithin, Sodium thioglycollate, Sodium thiosulphate and Sodium bisulphite. It is recommended for use in environmental testing, particularly in areas subjected to surface disinfection, and conforms to the requirements of the British Pharmacopeia for sterility testing of appropriate pharmaceutical products. The product also conforms to ISO 21149 to act as a diluent in the method for the enumeration of aerobic bacteria from cosmetic samples. Lecithin and Polysorbate 80 (Tween 80) inactivate surface disinfectants (Lecithin neutralises quaternary ammonium compounds and Tween 80 neutralises phenols, formalin, hexachlorophene and in combination with the Lecithin ethanol). Sodium thioglycollate neutralises mercurials, Sodium thiosulphate neutralises iodine and chlorine and Sodium bisulphite neutralises aldehydes. Bromocresol purple is incorporated into the medium to indicate glucose utilisation. -
For in vitro diagnostic use. BM0650 Deionised Water is used as a diluent for, where required, the preparation of clinical specimens and microbiological samples, the dilution of microorganisms and reconstitution of lyophilised antibiotic supplements during culture media preparation. Deionised Water is purified water that can be used as a general-purpose diluent in many areas of the laboratory. It may be used for creating 0.5 McFarland suspensions of bacterial isolates for identification tests or processing clinical specimens or industrial samples prior to microbiological examination. -
For in vitro diagnostic use. BM0655 Distilled Water can be used in the preparation of bacterial suspensions and as a diluent in areas of the laboratory. Distilled Water is manufactured from the distillation of deionised water. During this process almost all the impurities in the water are removed and this is why distilled water is chosen for specific laboratory applications. -
E.E. Broth is recommended as an enrichment medium when examining food and feedstuffs for Enterobacteriaceae. It is a modification of Brilliant Green Bile Broth, with an improved buffering capacity to encourage early growth and prevent autosterilization. E.E. Broth uses glucose instead of lactose to make the medium a test for all enterobacteria including non-lactose fermenting organisms. This formulation complies with the Harmonized USP/EP/JP. -
This broth is used to aid the rinsing of the membrane filter apparatus when performing sterility testing. This formulation complies with the United States Pharmacopoeia. -
This broth is used to aid the rinsing of the membrane filter apparatus when performing sterility testing. Fluid D has been formulated with the addition of polysorbate 80 to enable the rinsing of products containing lecithin or oil. This formulation complies with the Harmonised USP/EP/JP.
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This medium is intended for use in sterility testing of substances in accordance with the United States Pharmacopoeia and European Pharmacopoeia. It is a general purpose medium for the cultivation of both fastidious anaerobic and aerobic micro-organisms. Sodium Thioglycollate and L-cystine are included to reduce the medium to ensure anaerobiosis and to inactivate mercury and other heavy metallic compounds. A small amount of agar is added to further reduce the update of oxygen. However, in the event of oxidation, the medium will turn pink due to the presence of the resazurin. Providing only 30% of the medium has turned pink, the Eh may be restored by heating the medium (once only) in a boiling water bath. -
Product description to follow.
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Letheen Broth with Neutraliser and 1% Tween 80 is primarily intended for use in assessing the bactericidal activity of quaternary ammonium compounds and determining the phenol coefficient of cationic surfactants. It can also be used in environmental testing, particularly in areas subjected to surface disinfection. Lecithin and polysorbate 80 (Tween 80) inactivate surface disinfectants (lecithin neutralises quaternary ammonium compounds and Tween 80 neutralises phenols, formalin, hexachlorophene and in combination with the lecithin ethanol).
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This is a selective medium for the isolation of ESBL (Extended Spectrum Beta-Lactamase) producing strains of Escherichia coli. It should be noted that AmpC isolates may also be detected on this medium whilst non - ESBL organisms will be inhibited on this medium. The inclusion of bromocresol purple indicator displays the acid production due to the lactose fermentation by means of a colour change from purple to yellow. N.B. – This is double strength broth.
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A selective medium for the enrichment of Enterobacteriaceae and the detection of Escherichia coli in pharmaceutical products. The inclusion of Bromocresol Purple indicator makes the colour change caused by acid production from the fermentation of lactose easy to read with gas formation. The presence of Ox bile helps to suppress the growth of Gram positive and non-enteric bacterial species. This formulation complies with the Harmonized USP/EP/JP.
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Maximum Recovery Diluent (Peptone/ Saline Diluent) An osmotically controlled solution for the preparations of suspensions of food samples and for use as a diluent in dilution techniques for bacterial enumeration. The presence of a low level peptone lessens the physiological shock normally experienced by bacterial cells when they are introduced to a diluent such as Ringers Solution. The level of peptone is such that multiplication of the organisms is not possible in the time in which the sample will be present in the diluent. -
This is a liquid medium for growing pure cultures of Mycobacterium spp., including M. tuberculosis, for use in antimicrobial assays and biochemical tests. The medium is complex but includes L-Glutamic acid, ammonium sulphate, sodium citrate, pyridoxine and biotin as growth factors as well as magnesium sulphate and ferric ammonium citrate as sources of trace ions. Di-sodium phosphate and Mono-potassium phosphate are also present to maintain the pH of the medium. The medium is further enriched by the addition of the Middlebrook OADC Enrichment supplement. OADC contains oleic acid to provide fatty acids for growth promotion, bovine albumin and catalase as protective compounds as well as sodium chloride and dextrose.
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This medium is recommended by BSI and ISO for the enumeration of viable organisms in milk and other dairy products. It can also be used as a general-purpose medium for the cultivation of most organisms, particularly those that are less fastidious in their nutritional requirements.
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Modified Semi Solid Rappaport Vassiliadis Medium (MSRV) is a modification of Rappaport-Vassiliadis enrichment broth for detecting motile Salmonella spp. in faeces and food products. The original research on MSRV Medium revealed a semi-solid could be used as a rapid and sensitive test for isolating motile Salmonella spp. from food products following pre-enrichment or selective enrichment. The semi-solid medium allows motility to be detected as halos of growth around the original point of inoculation.
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This is a solution of novobiocin used as the selective agent in Modified Semi Solid Rappaport Vassiliadis Medium (BM4031) for the detection of motile Salmonella spp.
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This is a non-nutrient medium based on Page’s Amoeba Saline, a buffered salt solution, solidified with 1.5% Agar.
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This is a selective enrichment broth for the isolation of Salmonella spp. from pharmaceutical, food, dairy and environmental samples. Malachite Green and Magnesium Chloride are included in the formulation as selective agents due to their ability to inhibit most enteric organisms whilst allowing Salmonella spp. to multiply freely. Gram +ve bacteria and most other enteric bacteria, are typically susceptible to or inhibited by Malachite Green, the high osmotic pressure and/or the low pH of the medium. It should be noted that S.typhi and S.choleraesuis are sensitive to Malachite Green and may therefore be inhibited. This medium conforms to the requirements of the Harmonised USP/EP/JP. -
An osmotically controlled solution for the preparation of suspensions of food samples and for use as a diluent in dilution techniques for bacterial enumeration. The solution may also be used in the sampling of food production apparatus by the rinse and swab method. -
This is a selective medium for the isolation of yeasts and fungi and is particularly suitable for use in sterility testing. The low pH (5.6) of the medium is inhibitory to most bacteria and many diagnostic features such as spores and pigmentation are well developed on this medium. The formulation of this medium conforms to the requirements of the Harmonized USP/EP/JP. -
Saline (0.85%) is suitable for use in preparation of food samples and/or as a rinse fluid during examination of equipment etc. prior to culture. It can also be used as a general purpose diluent in many areas of the laboratory. -
BM0385 Saline (0.85%) with Beads is used in preparation of tissue specimens for microbiological examination prior to culture. It may also be used as a general-purpose diluent in many areas of the laboratory. Saline (0.85%) is recommended for processing clinical specimens for microbiological examination. The addition of glass beads will allow the specimen to be broken down and, therefore, aiding with the release of microorganisms into the saline for further testing. BM0385 Saline (0.85%) with Beads maintains the osmotic balance of the medium thereby maintaining cell morphology and integrity of bacteria. Glass beads assist with homogenisation of specimens prior to subculturing to primary isolation agar plates and/or enrichment broths. The solution does not interfere with any biochemical reaction and/or antibiotic susceptibility test. -
BM0397 Saline Tryptone Water (pH 7.0) is isotonic saline containing 0.1% tryptone that may be used as a general-purpose diluent in many areas of the laboratory. It is recommended by The British Standards Institute (1) and is tested according to the principles of ISO 11133:2014 (2). The product does not interfere with biochemical reactions used to identify organisms, such as indole production.
References
- The British Standards Institution (2015) BS EN 16615:2015 Chemical disinfectants and antiseptics. Quantitative test method for the evaluation of bactericidal and yeasticidal activity on non-porous surfaces with mechanical action employing wipes in the medical area (4- field test). Test method and requirements (phase 2, step 2). Published by BSI Standards Limited.
- International Organization for Standardization (2014) 11133:2014 Microbiology of food, animal feed and water - Preparation, production, storage and performance testing of culture media. Geneva, ISO.
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This medium is utilised for the transportation and cryopreservation of Streptococcus pneumoniae & Neisseria meningitidis isolates.
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This is a medium that can be used to differentiate between some of the Enterobacteriacae on the basis of four reactions, fermentation of Lactose, Glucose and Sucrose and the production of H2S. For use the medium is inoculated using a pure culture of the test organism which should be smeared onto the surface of the slope and stabbed into the butt of the medium. For details of the many reactions that may arise during the use of this medium reference should be made to one of the many standard textbooks.
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This is a plate count agar originally suggested by the American Public Health Association for the estimation of total viable counts in food and dairy products.
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BM1436 Tryptone Soya Agar (pH 7.2) is used for a wide range of applications, including culture storage, enumeration of cells, isolation of pure cultures, or general culture. It has been found to be useful in cosmetic testing, water, and wastewater applications. This medium meets the requirements of The British Standards Institution (1) and is tested according to the principles of ISO 11133:2014 (2). and is based on the original formulation described by Leavitt et al. in 1955 (3). In environmental monitoring applications, it is common for plates to be incubated at 30-35°C for bacterial colonies and 20-25°C for mould and fungi (4).
- The British Standards Institution (2015) BS EN 16615:2015 Chemical disinfectants and antiseptics. Quantitative test method for the evaluation of bactericidal and yeasticidal activity on non-porous surfaces with mechanical action employing wipes in the medical area (4- field test). Test method and requirements (phase 2, step 2). Published by BSI Standards Limited.
- International Organization for Standardization (2014) 11133:2014 Microbiology of food, animal feed and water – Preparation, production, storage and performance testing of culture media. Geneva, ISO.
- Leavitt, J., Naidorf, I. and Shugaevsky, P. (1955) Aerobes and anaerobes in endodontics. Part I. The undetected anaerobes in endodontics. Part II. Sensitive culture medium for the detection of both aerobes and anaerobes. NYSDJ, 25, pp.377-382.
4. Anon. (1987) Testing methods for use in quality assurance of culture media. Int. J. Food Microbiol., 5, pp.291-296
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This is a general-purpose complex medium for the cultivation and isolation of aerobic and anaerobic microorganisms. The base medium, Tryptone Soya Agar (Soybean-Casein Digest agar), conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EP), and Japanese Pharmacopoeia (JP). The medium can be incubated under aerobic or anaerobic conditions after use for sterility testing, air sampling and other areas of bacteriological investigation. -
This is a general-purpose complex medium for cultivation and isolation of fastidious bacteria, yeasts and moulds. The formulation is based on the United States Pharmacopoeia (USP Medium II) and European Pharmacopoeia (EP Medium B). Lecithin and Polysorbate 80 (Tween 80) are added to inactivate surface disinfectants. The medium can be incubated under aerobic or anaerobic conditions for sterility testing, air sampling and other areas of bacteriological investigation. -
Tryptone Soya Broth is also commonly referred to as Soybean-Casein Digest Medium or Tryptic Soya Broth and is abbreviated as TSB. This is a general purpose and nutritious medium for the non-selective enrichment of non-fastidious and some fastidious organisms. Tryptone and Soy peptone are the nitrogen sources in the medium. Glucose is the carbon energy source that facilitates organism growth. Sodium chloride maintains osmotic balance; Di-potassium phosphate is a buffering agent to prevent auto-sterilisation due to acid production during the growth of some organisms. Tryptone Soya Broth formulation conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP). -
This is a general-purpose broth for cultivation of fastidious bacteria, yeasts and moulds. The medium incorporates Polysorbate 80 to act as an emulsifying agent and inactivate phenols. Lecithin is also incorporated to inactivate quaternary ammonium compounds. The medium can be incubated under aerobic or anaerobic conditions for sterility testing, and other areas of bacteriological investigation. -
Tryptone Water is an alternative medium to Peptone Water and more reliable for the testing of Indole production. The medium has a high content of Tryptophan that many organisms, particularly coliforms, break down to form Indole. After incubation add a few drops of Indole reagent to determine the Indole reaction (Red colour is Positive). -
Based on Christensen’s Medium this medium is generally used to detect rapid urease activity of Proteus spp although it can be used to detect urease activity of other Enterobacteriaceae. When used for the later purpose it is necessary to increase the incubation time to as long as 48 hours.