SKU: KM0052 Category:

This medium is based on the formulation published by Platt, Atherton & Simpson1 and is used for the cultivation of Taylorella equigenitalis, the causative organism in contagious equine metritis.
It is routinely used for culturing swabs taken from the genitalia of mares and stallions.
Enzymatic digest of casein and soy peptone supply nitrogen, carbon and vitamins and L-cystine is a required growth factor.
Sodium chloride provides osmotic balance and sodium sulphite is present as a reducing agent.
The medium is made selective by the addition of CEMO Selective Supplement (LS0041) to control bacteria and fungi from swab samples.

1. Platt, H., Atherton, J. G. and Simpson, D. J. 1978. Equine Vet J 10, 153–159.

Additional Information


Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Recommended Incubation

Incubate at 37°C ± 1°C in 5-10% CO2 for 24-48 hours

Product Description

Organisms Ref. No Result
Taylorella equigenitalis ATCC 35865 Growth, yellow / cream colonies
Taylorella equigenitalis NCTC 11225 Growth, yellow / cream colonies
Escherichia coli ATCC 25922 Inhibited
Staphylococcus aureus ATCC 25933 Inhibited


Recommended incubation: Incubate at 37°C ± 1°C in 5-10% CO2 for 24-48 hours.


Enzymatic digest of casein15
Soy peptone5
Sodium chloride5
Sodium sulphite0.2


Enzymatic digest of casein 15.0
Soy peptone 5.0
Sodium chloride 2.0
L-cystine 0.3
Sodium sulphite 0.2
Agar 12.0
Total 37.5
*Adjusted/supplemented as required to meet performance requirements



Suspend 37.5 g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes.

Sterilise at 121°C for 15 minutes with frequent stirring to completely dissolve the medium. Cool to 45- 50°C. Aseptically add 5-7% of defibrinated horse blood and mix well. Heat the medium to 80°C with frequent stirring until the media become a chocolate brown colour. Cool to 45- 50°C and aseptically add 2 vials of CEMO selective supplement (LS0041). Mix well and aseptically dispense into appropriate sterile containers.